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2713 results for " Mitochondrion membrane" - showing 1250-1300
GATM, Polyclonal Antibody (Cat# AAA8551247)
Full Name
GATM (N-Term) Rabbit pAb
Gene Names
GATM; AT; AGAT; CCDS3
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa.)
COX-4, Polyclonal Antibody (Cat# AAA8000085)
Full Name
COX-4 Antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of SOD2 expression in Rat brain lysate (MBS179281).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOD2 )
SOD2/Mnsod, Monoclonal Antibody (Cat# AAA179281)
Full Name
Anti-SOD2/Mnsod Rabbit Monoclonal Antibody
Gene Names
SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cytochrome C expression in HeLa cell lysate (MBS179971).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYCS )
Cytochrome C, Monoclonal Antibody (Cat# AAA179971)
Full Name
Anti-Cytochrome C Rabbit Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Bid expression in Jurkat cell lysate (MBS179430).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BID monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BID )
Bid, Monoclonal Antibody (Cat# AAA179430)
Full Name
Anti-Bid Rabbit Monoclonal Antibody
Gene Names
BID; FP497
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate (MBS1750175).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR, Monoclonal Antibody (Cat# AAA1750175)
Full Name
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of p53 (acetyl K382) expression in Jurkat cell lysate treated with etopside and TSA (MBS1750168).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP53 )
p53, Monoclonal Antibody (Cat# AAA1750168)
Full Name
Anti-p53 (acetyl K382) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa.)
COX-4, Polyclonal Antibody (Cat# AAA8000089)
Full Name
COX-4 Antibody: ATTO 594
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa.)
COX-4, Polyclonal Antibody (Cat# AAA8000096)
Full Name
COX-4 Antibody: Biotin
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa.)
COX-4, Polyclonal Antibody (Cat# AAA8000097)
Full Name
COX-4 Antibody: FITC
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Cytochrome C expression in Human kidney lysate (MBS179972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYCS )
Cytochrome C, Monoclonal Antibody (Cat# AAA179972)
Full Name
Anti-Cytochrome C Rabbit Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Bcl-2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179054).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCL2 )
Bcl-2, Monoclonal Antibody (Cat# AAA179054)
Full Name
Anti-Bcl-2 Rabbit Monoclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Src expression in U87-MG cell lysate (MBS179112).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRC )
Src, Monoclonal Antibody (Cat# AAA179112)
Full Name
Anti-Src Rabbit Monoclonal Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-Raf1 (S621) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate (MBS1750233).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAF1 )
Raf1, Monoclonal Antibody (Cat# AAA1750233)
Full Name
Anti-Phospho-Raf1 (S621) Rabbit Monoclonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody . Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
FUNDC1, Polyclonal Antibody (Cat# AAA809681)
Full Name
FUNDC1 Antibody: Biotin
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-774). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-774) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): ~25 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001376)
Full Name
BAD Antibody: HRP
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of StAR using anti-StAR antibody (MBS1750318).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD. )
StAR, Polyclonal Antibody (Cat# AAA1750318)
Full Name
Anti-StAR Picoband Antibody
Gene Names
STAR; STARD1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of p53 (acetyl K370) expression in HeLa cell lysate treated with Trichostatin A (MBS1750167).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP53 )
p53, Monoclonal Antibody (Cat# AAA1750167)
Full Name
Anti-p53 (acetyl K370) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Structure
Visomitin, Inhibitor (Cat# AAA5750032)
Full Name
Visomitin
Purity
95.4%
Pricing
$195/5 mg | $215/10 mg | $265/25 mg | $330/50 mg | $480/100 mg | $205/1 mL (in DMSO) | $1,900/5x100 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-MCL 1 Polyclonal Antibody . Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-MCL 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
MCL 1, Polyclonal Antibody (Cat# AAA8001678)
Full Name
MCL 1 Antibody: Alkaline Phosphatase
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Src expression in Hela cell lysate (MBS179111).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRC )
Src, Monoclonal Antibody (Cat# AAA179111)
Full Name
Anti-Src Rabbit Monoclonal Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CDK1 in HeLa cell lysate (MBS179188).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1 )
CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179188)
Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-p53 (Ser9) expression in (1)HepG2 cell lysates treated with etoposide; (2)HeLa cell lysates treated with etoposide (MBS1750169).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP53 )
p53, Monoclonal Antibody (Cat# AAA1750169)
Full Name
Anti-Phospho-p53 (S9) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human stomach, using MCL1 Antibody(MBS179422)MCL1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MCL1 Antibody (MBS179422)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
MCL1, Monoclonal Antibody (Cat# AAA179422)
Full Name
Anti-MCL1 Rabbit Monoclonal Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody(MBS1750172)TP53 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TP53 Antibody (MBS1750172)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
p53, Monoclonal Antibody (Cat# AAA1750172)
Full Name
Anti-Phospho-p53 (S392) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot detection of Hexokinase 1 in Jurkat, HepG2 and Hela cell lysates using Hexokinase 1 mouse mAb (dilution 1:500).Predicted band size:102KDa.Observed band size:102KDa.)
Hexokinase 1, Monoclonal Antibody (Cat# AAA475613)
Full Name
Anti-Hexokinase 1 Mouse mAb
Gene Names
HK1; HK; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-Mitofusin 2 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Mitofusin 2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)
Mitofusin 2, Polyclonal Antibody (Cat# AAA8000043)
Full Name
Mitofusin 2 Antibody: FITC
Gene Names
MFN2; HSG; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa.)
COX-4, Polyclonal Antibody (Cat# AAA8000094)
Full Name
COX-4 Antibody: Alkaline Phosphatase
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-Mitofusin 2 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Mitofusin 2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)
Mitofusin 2, Polyclonal Antibody (Cat# AAA8000044)
Full Name
Mitofusin 2 Antibody: HRP
Gene Names
MFN2; HSG; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of GARS expression in (1) Jurkat cell lysate; (2) 293T cell lysate (MBS1750025).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GARS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GARS )
GARS, Monoclonal Antibody (Cat# AAA1750025)
Full Name
Anti-GARS Rabbit Monoclonal Antibody
Gene Names
GARS; HMN5; CMT2D; DSMAV; GlyRS; SMAD1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-MCL 1 Polyclonal Antibody . Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-MCL 1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
MCL 1, Polyclonal Antibody (Cat# AAA8001670)
Full Name
MCL 1 Antibody: ATTO 390
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (MBS1750313).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysate,Lane 2: mouse thymus tissue lysate,Lane 3: human MCF-7 whole Cell lysate,Lane 4: human 22RV1 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bcl-2 at approximately 29KD. The expected band size for Bcl-2 is at 26KD. )
Bcl-2, Polyclonal Antibody (Cat# AAA1750313)
Full Name
Anti-Bcl-2 Picoband Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of mTOR expression in Jurkat cell lysate (MBS179041).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR/Tor, Monoclonal Antibody (Cat# AAA179041)
Full Name
Anti-mTOR/Tor Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-773). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-773) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): 28 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001357)
Full Name
BAD Antibody: FITC
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen, using Bcl-2 Antibody(MBS179053)BCL2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL2 Antibody (MBS179053)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Bcl-2, Monoclonal Antibody (Cat# AAA179053)
Full Name
Anti-Bcl-2 Rabbit Monoclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of PDK2 using anti-PDK2 antibody (MBS1750824).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,Lane 2: rat skeletal muscle tissue lysates,Lane 3: rat brain tissue lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse heart tissue lysates,Lane 6: mouse skeletal muscle tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDK2 at approximately 46KD. The expected band size for PDK2 is at 46KD. )
PDK2, Polyclonal Antibody (Cat# AAA1750824)
Full Name
Anti-PDK2 Picoband antibody
Gene Names
PDK2; PDHK2; PDKII
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A (MBS1750234).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAF1 )
Raf1, Monoclonal Antibody (Cat# AAA1750234)
Full Name
Anti-Phospho-Raf1 (S259) Rabbit Monoclonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Raf1 using anti-Raf1 antibody (MBS1750407).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysate,Lane 2: mouse kidney tissue lysate,Lane 3: human MCF-7 whole cell lysate,Lane 4: human 22RV1 whole cell lysate,Lane 5: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raf1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Raf1 at approximately 73KD. The expected band size for Raf1 is at 73KD. )
Raf1, Polyclonal Antibody (Cat# AAA1750407)
Full Name
Anti-Raf1 Picoband Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
ABL, Polyclonal Antibody (Cat# AAA8554080)
Full Name
ABL (Phospho-Tyr204) Rabbit pAb
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; BCR-ABL; bcr/abl
Reactivity
Human
Applications
ICC, DB
Purity
Affinity Purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-774). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-774) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): ~25 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001374)
Full Name
BAD Antibody: Biotin
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~18.3 kDa BAD protein using Rabbit Anti-BAD Polyclonal Antibody (SPC-774). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-BAD Polyclonal Antibody (SPC-774) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~18.3 kDa. Other Band(s): ~25 kDa (PTM).)
BAD, Polyclonal Antibody (Cat# AAA8001367)
Full Name
BAD Antibody: ATTO 594
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CDK1 expression in HeLa cell lysate (MBS179190).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1 )
CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179190)
Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DNAJC15 Antibody(MBS1750106)DNAJC15 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DNAJC15 Antibody (MBS1750106)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
DNAJC15, Monoclonal Antibody (Cat# AAA1750106)
Full Name
Anti-DNAJC15 Rabbit Monoclonal Antibody
Gene Names
DNAJC15; MCJ; HSD18; DNAJD1
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CDK1 expression in (1)A431 cell lysate;(2)HeLa cell lysate (MBS179191).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDK1 )
CDK1/Cdc2, Monoclonal Antibody (Cat# AAA179191)
Full Name
Anti-CDK1/Cdc2 Rabbit Monoclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-p53 (S376) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid (MBS1750173).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP53 )
p53, Monoclonal Antibody (Cat# AAA1750173)
Full Name
Anti-Phospho-p53 (S376) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of PDK1 using anti-PDK1 antibody (MBS1750604).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human COLO-320 whole cell lysates,Lane 2: human PANC-1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDK1 at approximately 58KD. The expected band size for PDK1 is at 49KD. )
PDK1, Polyclonal Antibody (Cat# AAA1750604)
Full Name
Anti-PDK1 Picoband antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of LRRK2 in HEK293 cell lysate transfected with 3*DYKDDDDK wild type, full length LRRK2 (MBS179199).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRK2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRK2 )
LRRK2, Monoclonal Antibody (Cat# AAA179199)
Full Name
Anti-LRRK2 Rabbit Monoclonal Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer, using RPS3 Antibody(MBS179685)RPS3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RPS3 Antibody (MBS179685)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
RPS3/Ribosomal Protein S3, Monoclonal Antibody (Cat# AAA179685)
Full Name
Anti-RPS3/Ribosomal Protein S3 Rabbit Monoclonal Antibody
Gene Names
RPS3; S3
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
BELCIN 1, Polyclonal Antibody (Cat# AAA8554164)
Full Name
BELCIN 1 Rabbit pAb
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
DB
Purity
Affinity Purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-FUNDC1 Polyclonal Antibody . Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-FUNDC1 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
FUNDC1, Polyclonal Antibody (Cat# AAA809670)
Full Name
FUNDC1 Antibody
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
