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10000 results for "Transcription Factor ELISA" - showing 1000-1050


Western Blot (WB) (Host: RabbitTarget Name: RPA1Sample Type: Human 721_BAntibody Dilution: 1.0ug/mlRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

RPA1, Polyclonal Antibody (Cat# AAA3212832)

Full Name
RPA1 antibody - middle region
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Western blot analysis of GATA2 in lysates of COS7 , using GATA2 Antibody.)

GATA2, Polyclonal Antibody (Cat# AAA9611057)

Full Name
GATA2 Antibody
Gene Names
GATA2; DCML; IMD21; NFE1B; MONOMAC
Reactivity
Human
Predicted: Bovine, Horse, Rabbit, Dog
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Western Blot (WB) (Western blot analysis of PROP1 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

PROP1, Polyclonal Antibody (Cat# AAA9604123)

Full Name
PROP1 Antibody
Gene Names
PROP1; CPHD2; PROP-1
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of DP-2 using HeLa whole cell lysates)

DP-2, Polyclonal Antibody (Cat# AAA9608850)

Full Name
DP-2 Antibody
Gene Names
TFDP2; DP2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of mouse lung, using NKX2-1antibody. The lane on the left is treated with the antigen-specific peptide.)

NKX2-1, Polyclonal Antibody (Cat# AAA9607833)

Full Name
NKX2-1 Antibody
Gene Names
NKX2-1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; NMTC1; T/EBP; TITF1; TTF-1; NKX2.1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Rabbit Anti-SF1 antibodyParaffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

SF1, Polyclonal Antibody (Cat# AAA3205717)

Full Name
SF1 antibody - N-terminal region
Gene Names
SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Protein A purified
Pricing
$390/0.1 mL | $1,750/5x0.1 mL
Western Blot (WB) (All lanes: Transcription intermediary factor 1-beta antibody at at 2 ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 92 kDa Observed band size: 92 kDa Immunohistochemistry)

Transcription intermediary factor 1-beta, Polyclonal Antibody (Cat# AAA715816)

Full Name
Rabbit anti-human Transcription intermediary factor 1-beta polyclonal Antibody, HRP conjugated
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB) (Western blot analysis of TFAP2D expression in TFAP2D transfected 293T cell lysate, The lane on the left is treated with the antigen-specific peptide.)

TFAP2D, Polyclonal Antibody (Cat# AAA9603935)

Full Name
TFAP2D Antibody
Gene Names
TFAP2D; TFAP2BL1
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of TCEAL8 using K562 whole cell lysates)

TCEAL8, Polyclonal Antibody (Cat# AAA9610286)

Full Name
TCEAL8 Antibody
Gene Names
TCEAL8; WEX3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9607689 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MEF2C, Polyclonal Antibody (Cat# AAA9607689)

Full Name
MEF2C Antibody
Gene Names
MEF2C; DEL5q14.3; C5DELq14.3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot: Sample: Recombinant GATA4, Rat.)

GATA Binding Protein 4 (GATA4), Polyclonal Antibody (Cat# AAA2004291)

Full Name
Polyclonal Antibody to GATA Binding Protein 4 (GATA4)
Reactivity
Rat
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $340/0.5 mL | $415/1 mL | $1,205/5x1 mL
Western Blot (WB) (Western blot analysis of extracts of K562, using USF1 antibody. The lane on the left is treated with the antigen-specific peptide.)

USF1, Polyclonal Antibody (Cat# AAA9607248)

Full Name
USF1 Antibody
Gene Names
USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis NR5A2 using COS7 whole cell lysates)

NR5A2, Polyclonal Antibody (Cat# AAA9608815)

Full Name
NR5A2 Antibody
Gene Names
NR5A2; B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis SOX1 using HeLa whole cell lysates)

SOX1, Polyclonal Antibody (Cat# AAA9608616)

Full Name
SOX1 Antibody
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot: Sample: Rat Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TFAM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Transcription Factor A, Mitochondrial (TFAM), Polyclonal Antibody (Cat# AAA2002803)

Full Name
Polyclonal Antibody to Transcription Factor A, Mitochondrial (TFAM)
Gene Names
Tfam; Mttfa
Reactivity
Rat
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$170/0.1 mL | $215/0.2 mL | $370/0.5 mL | $455/1 mL | $1,285/5x1 mL
Western Blot (WB) (Western blot analysis of Jurkat whole cell lysates, using PDX1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

PDX1, Polyclonal Antibody (Cat# AAA9607819)

Full Name
PDX1 Antibody
Gene Names
PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PAGEN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Chromatin Immunoprecipitation (ChIP) (Chromatin Immunoprecipitation (ChIP) Using SMARCA1 antibody - N-terminal region and HCT116 Cells)

SMARCA1, Polyclonal Antibody (Cat# AAA3203964)

Full Name
SMARCA1 antibody - N-terminal region
Gene Names
SMARCA1; SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; hSNF2L; NURF140
Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
ChIP, IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
SDS-Page (SDS-PAGE Analysis of Purified SOX9 Monoclonal Antibody (rSOX9/2288). Confirmation of Integrity and Purity of the Antibody.)

SOX9/SRY-box 9, Antibody (Cat# AAA4381259)

Full Name
SOX9/SRY-box 9
Gene Names
SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10
Reactivity
Human
Applications
EIA, IP, WB
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$375/0.1 mg (With BSA & Azide at 0.2mg/mL) | $375/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL)
Western Blot (WB) (Western blot analysis of CREB expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

CREB, Polyclonal Antibody (Cat# AAA9601792)

Full Name
CREB Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT protein family. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), form homo- or heterodimers, and translocate to the cell nucleus where they act as transcription activators. Besides, Epidermal Growth Factor Receptor 2 (EGFR2) has been identified as an interactor of STAT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human STAT3 and recombinant human EGFR2. Briefly, STAT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EGFR2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-STAT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of STAT3 and EGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of STAT3 with EGFR2)

Signal Transducer And Activator Of Transcription 3 (STAT3), Active Protein (Cat# AAA2105149)

Full Name
Active Signal Transducer And Activator Of Transcription 3 (STAT3)
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $465/0.05 mg | $715/0.1 mg | $885/0.2 mg | $1,725/0.5 mg | $2,575/1 mg | $6,290/5 mg
Western Blot (WB) (Western blot analysis on HepG2 cell lysate using E2F6 Antibody, The lane on the left is treated with the antigen-specific peptide.)

E2F6, Polyclonal Antibody (Cat# AAA9600090)

Full Name
E2F6 Antibody
Gene Names
E2F6; E2F-6
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601072 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

STAT5A, Polyclonal Antibody (Cat# AAA9601072)

Full Name
Phospho-STAT5A (Ser780) Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of PAX5 using anti-PAX5 antibody (MBS1750462).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAX5 at approximately 45KD. The expected band size for PAX5 is at 42KD. )

PAX5/Bsap, Polyclonal Antibody (Cat# AAA1750462)

Full Name
Anti-PAX5/Bsap Picoband antibody
Gene Names
PAX5; ALL3; BSAP
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF) (MBS9601940 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

AML1, Polyclonal Antibody (Cat# AAA9601940)

Full Name
AML1 Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of anti-IRF5 Antibody (N-term) MBS9213786 in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.)

IRF5, Polyclonal Antibody (Cat# AAA9213786)

Full Name
IRF5 Antibody (N-term)
Gene Names
IRF5; SLEB10
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Host: MouseTarget Name: SOX1Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

SOX1, Polyclonal Antibody (Cat# AAA3200569)

Full Name
SOX1 antibody - middle region
Reactivity
Cow, Dog, Human, Mouse, Rabbit, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Western blot analysis of MIBP1 expression in immunising recombinant protein, The lane on the left is treated with the antigen-specific peptide.)

MIBP1, Polyclonal Antibody (Cat# AAA9603913)

Full Name
MIBP1 Antibody
Gene Names
HIVEP2; SHN2; ZAS2; MBP-2; MIBP1; MRD43; ZNF40B; HIV-EP2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (Western Blot analysis using MESP1 antibodyWestern Blot showing MESP1 antibody used against MESP1 (AA: 1-200)-hIgGFc transfected HEK293 cell lysate.)

MESP1, Monoclonal Antibody (Cat# AAA533274)

Full Name
MESP1 antibody
Gene Names
MESP1; bHLHc5
Applications
EIA, WB
Pricing
$565/0.1 mL | $2,540/5x0.1 mL
Western Blot (WB) (Western blot analysis of CCNT2 expression in HepG2 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

CCNT2, Polyclonal Antibody (Cat# AAA9603822)

Full Name
CCNT2 Antibody
Gene Names
CCNT2; CYCT2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Host: MouseTarget Name: THRASample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

THRA, Polyclonal Antibody (Cat# AAA3202288)

Full Name
THRA antibody - middle region
Gene Names
THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Host: RatTarget Name: SMAD5Sample Tissue: Rat LiverAntibody Dilution: 1ug/ml)

SMAD5, Polyclonal Antibody (Cat# AAA3200703)

Full Name
SMAD5 antibody - middle region
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemistry with Human Pancrease lysate tissue at an antibody concentration of 5.0ug/ml using anti-TEAD4 antibody )

TEAD4, Polyclonal Antibody (Cat# AAA3203994)

Full Name
TEAD4 antibody - C-terminal region
Gene Names
TEAD4; TEF3; RTEF1; TEF-3; EFTR-2; TEFR-1; TCF13L1; hRTEF-1B
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunofluorescence (IF) (Sample Type :Mouse retina Primary Antibody Dilution :1:200 Secondary Antibody :Donkey anti rabbit-Cy3Secondary Antibody Dilution :1:1000 Color/Signal Descriptions :Red: PPARGC1AGene Name :PPARGC1ASubmitted by :Dr. Joshua Sanes )

PPARGC1A, Polyclonal Antibody (Cat# AAA3200467)

Full Name
PPARGC1A antibody - N-terminal region
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IF, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Western blot analysis of TCF7L2 using COLO205 whole lysates.)

TCF7L2, Polyclonal Antibody (Cat# AAA9608220)

Full Name
TCF7L2 Antibody
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using FOXB1/2 antibody.)

FOXB1/2, Polyclonal Antibody (Cat# AAA9604872)

Full Name
FOXB1/2 Antibody
Gene Names
FOXB1; FKH5; HFKH-5
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (HIF1a (Hypoxia-inducible factor 1-alpha) is a subunit of a transcription factor, which functions as a master transcriptional regulator of the adaptive response to hypoxia. The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors, including HIF1a, thus a binding ELISA assay was conducted to detect the interaction of HIF1a and HSP90. Briefly, recombinant human HIF1a were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP90-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HIF1a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of HIF1a and HSP90 was shown in Figure 1, and this effect was in a dose dependent manner.)

Hypoxia Inducible Factor 1 Alpha (HIF1a), Active Protein (Cat# AAA2097345)

Full Name
Active Hypoxia Inducible Factor 1 Alpha (HIF1a)
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunofluorescence (IF) (MBS9608573 staining HepG2  cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

FOXA1, Polyclonal Antibody (Cat# AAA9608573)

Full Name
FOXA1 Antibody
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of MCF-7 cell stained with TREF1 Antibody (C-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).TREF1 immunoreactivity is localized to Nucleus significantly.)

TREF1, Polyclonal Antibody (Cat# AAA9200686)

Full Name
TREF1 Antibody (C-term)
Gene Names
TRERF1; RAPA; BCAR2; TREP132; TReP-132; HSA277276; dJ139D8.5
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis on COLO205 cell lysate using ATF6B Antibody, The lane on the left is treated with the antigen-specific peptide.)

ATF6B, Polyclonal Antibody (Cat# AAA9600353)

Full Name
ATF6B Antibody
Gene Names
ATF6B; G13; CREBL1; CREB-RP
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of SOX6 Antibody expression in Hela cells lysates.The lane on the left is treated with the antigen-specific peptide.)

SOX6, Polyclonal Antibody (Cat# AAA9601390)

Full Name
SOX6 Antibody
Gene Names
SOX6; SOXD; HSSOX6
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA, ChIP
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601186 at 1/100 staining human colon carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

FKHR, Polyclonal Antibody (Cat# AAA9601186)

Full Name
Phospho-FKHR (Ser256) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of FKHR phosphorylation expression in EGF treated HeLa whole cell lysates)

FKHR, Polyclonal Antibody (Cat# AAA9601187)

Full Name
Phospho-FKHR (Ser319) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9605231 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

ALS2CR8, Polyclonal Antibody (Cat# AAA9605231)

Full Name
ALS2CR8 Antibody
Gene Names
CARF; ALS2CR8; NYD-SP24
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of MEF2A phosphorylation expression in UV treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

MEF2A, Polyclonal Antibody (Cat# AAA9601151)

Full Name
Phospho-MEF2A (Thr319) Antibody
Gene Names
MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from HUVEC, using FOXC1/2 Antibody. Lane 1 was treated with the blocking peptide.)

FOXC1/2, Polyclonal Antibody (Cat# AAA9604714)

Full Name
FOXC1/2 Antibody
Gene Names
FOXC1; ARA; IGDA; IHG1; ASGD3; FKHL7; IRID1; RIEG3; FREAC3; FREAC-3
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600159 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

NM23, Polyclonal Antibody (Cat# AAA9600159)

Full Name
NM23 Antibody
Gene Names
NME1-NME2; NMELV; NM23-LV
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601526 at 1/100 staining Human spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Fos, Polyclonal Antibody (Cat# AAA9601526)

Full Name
FOS Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis NKX23 using RAW264.7 whole cell lysates)

NKX23, Polyclonal Antibody (Cat# AAA9609905)

Full Name
NKX23 Antibody
Gene Names
NKX2-3; CSX3; NK2.3; NKX2C; NKX2.3; NKX4-3
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.)

HES5, Polyclonal Antibody (Cat# AAA153653)

Full Name
HES5 Antibody
Gene Names
HES5; bHLHb38
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
HES5 antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB) (Western Blot analysis of COS-7 cells with Phospho-Stat1 (Tyr701) Polyclonal Antibody at dilution of 1:1000)

Stat1, Polyclonal Antibody (Cat# AAA2538690)

Full Name
Phospho-Stat1 (Y701) Polyclonal Antibody
Gene Names
Stat1; AA408197; 2010005J02Rik
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IP, EIA
Purity
Antigen Affinity Purified
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
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