The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data (SLC5A4 antibody used at 1.25ug/ml to detect target protein.)
Western Blot (WB) (Western Blot (WB) analysis of SLC5A2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)
Testing Data (SLC5A4 antibody used at 1.25ug/ml to detect target protein.)
Western Blot (WB) (Western Blot detection against Immunogen (31.61kD).)
Western Blot (WB) (Western Blot analysis of SLC5A2 expression in transfected 293T cell line by SLC5A2 monoclonal antibody. Lane 1: SLC5A2 transfected lysate (72.9kD). Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged SLC5A2 is 0.03ng/ml as a capture antibody.)
Wester Blot (WB) (Dilution: WB 1/500 - 1/1000Western blot analysis of SGLT2 expression in HEK293T (A), Hela (B), DLD (C), rat heart (D), rat liver (E), rat kidney (F) whole cell lysates.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SLC5A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SLC5A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using SLC5A2 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Immunofluorescence (IF) (Immunofluorescence analysis of rat kidney using SLC5A2 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of human kidney using SLC5A2 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
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