Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Western Blot (WB) (Western blot analysis of extracts from Hela cells untreated or treated with UV using CREB(Phospho-Ser133) Antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Phospho-Ser133) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)
Testing Data (Western blot analysis of extracts from Hela cells, untreated or treated with Ca2+, using HSP27 (Ab-78) antibody (Line 1 and 2) and HSP27 (Phospho- Ser78) antibody (Line 3 and 4).)
Western Blot (WB) (Western blot analysis of extracts from Hela cells using HSP27(Ab-15) Antibody and the same antibody preincubated with blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Ab-15) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-142) Antibody (left) or the same antibody preincubated with blocking peptide(right).)
Western Blot (WB) (Western blot analysis NDRG1 (Phospho-Thr346) using EGF treated 293 whole cell lysates)
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)
Western Blot (WB) (Western blot analysis of HSP27 expression in HeLa (A), Jurkat (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunocytochemistry (ICC) (Immunocytochemistry stain of Hela using HSPB1 antibody (1:300).)
Western Blot (WB) (Western blot detection of HSPB1 in Hela,HsHela,A431, COS7, LNCAP&MCF7 cell lysates and using HSPB1 antibody (1:1000 diluted). Predicted band size: 23KDa Observed band size:27KDa)
Immunofluorescence (IF) (Immunofluorescence analysis of COS7 cells, using NFAT5 (Phospho-Ser1197) antibody. NFAT5 (Phospho-Ser1197) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded MBS7046320 using human lung cancer at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7046320 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells using MBS7048424 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot (1:1000) analysis of HspB1 expression in Hella whole cell lysate with HspB1 antibody)
Western Blot (WB) (Western blot analysis of lysates from COLO205 cells, using ATF6B Antibody. The lane on the right is blocked with the synthesized peptide.)
Western Blot (WB) (Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~265.3 kDa CBP protein using Rabbit Anti-CBP Polyclonal Antibody (SPC-752). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-CBP Polyclonal Antibody (SPC-752) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~265.3 kDa.)
Western Blot (WB) (Western blot analysis of lysates from HeLa cells treated with forskolin 40nM 30', using NFAT5/TonEBP (Phospho-Ser155) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB) (Western blot analysis of HSP27 (pS82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of HSP27 (pS82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Western Blot (WB) (Western blot analysis of lysates from K562 cells, using OXSR1 Antibody. The lane on the right is blocked with the synthesized peptide.)
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