Rabbit anti-Human, Mouse NFAT5/TonEBP Polyclonal Antibody | anti-NFAT5/TonEBP antibody

NFAT5/TonEBP (Phospho-Ser155) Antibody

Cat. Num. AAA9503390

• Gene Names: NFAT5; NFATZ; OREBP; NF-AT5; NFATL1; TONEBP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: NFAT5/TonEBP; Polyclonal Antibody; NFAT5/TonEBP (Phospho-Ser155) Antibody; KIAA0827; NF-AT5; nuclear factor of activated T cells 5; T cell transcription factor NFAT5; TonE-binding protein; TonEBP; tonicity-responsive enhancer-binding protein; anti-NFAT5/TonEBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: NFAT5/TonEBP (Phospho-Ser155) Antibody detects endogenous levels of NFAT5/TonEBP only when phosphorylated at Ser155.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1,549

• Applicable Applications for anti-NFAT5/TonEBP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S155; M:S155
• Immunogen: The antiserum was produced against synthesized peptide derived from human NFAT5/TonEBP around the phosphorylation site of Ser155.
• Immunogen Range: 121-170
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells treated with forskolin 40nM 30', using NFAT5/TonEBP (Phospho-Ser155) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 164419746
• NCBI GeneID: 10725
• NCBI Accession #: NP_001106649.1
• NCBI GenBank Nucleotide #: NM_001113178.2
• UniProt Accession #: O94916; O95693; Q7LA65; Q969Q8; Q96QH3; Q9UN18; A2RRB4; A6H8V5; E9PHR7
• Molecular Weight: 165 kDa
• NCBI Official Full Name: nuclear factor of activated T-cells 5 isoform d
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 5
• NCBI Official Symbol: NFAT5
• NCBI Official Synonym Symbols: NFATZ; OREBP; NF-AT5; NFATL1; TONEBP
• NCBI Protein Information: nuclear factor of activated T-cells 5
• UniProt Protein Name: Nuclear factor of activated T-cells 5
• UniProt Gene Name: NFAT5
• UniProt Synonym Gene Names: KIAA0827; TONEBP; TonEBP

NCBI Description

The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed:10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867). Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394).