Rabbit ATF4 Polyclonal Antibody | anti-ATF4 antibody

ATF4 (Phospho-Ser245)

Cat. Num. AAA191065

• Gene Names: ATF4; CREB2; TXREB; CREB-2; TAXREB67
• Applications: Immunoblot, Western Blot, Immunohistochemistry
• Synonyms: ATF4; Polyclonal Antibody; ATF4 (Phospho-Ser245); anti-ATF4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous human ATF4 only when phosphorylated at serine 245.
• Form/Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.
• Sequence Length: 351

• Applicable Applications for anti-ATF4 antibody: Immunoblotting (IB), Immunohistochemistry (IHC)
• Application Notes: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~45kDa is detected.; Immunohistochemistry: use at dilution of 1:50- 1:100.; Dilute in PBS or medium that is identical to that used in the assay system.
• Antigen: Peptide sequence that includes phosphorylation sites of serine 245 (N-R-S(p)-L-P) derived from human ATF4 and conjugated to KLH.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Can be stored at 4 degree C for short-term use.

Immunohistochemistry (IHC)

Testing Data

Related Product Information for anti-ATF4 antibody

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA-binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Diseases associated with ATF4 include leukoencephalopathy with vanishing white matter and T-cell leukemia.

NCBI and Uniprot Product Information

• NCBI GI #: 33469976
• NCBI GeneID: 468
• NCBI Accession #: NP_001666.2
• NCBI GenBank Nucleotide #: NM_001675.4
• UniProt Accession #: P18848; Q9UH31
• Molecular Weight: 38,590 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-4
• NCBI Official Synonym Full Names: activating transcription factor 4
• NCBI Official Symbol: ATF4
• NCBI Official Synonym Symbols: CREB2; TXREB; CREB-2; TAXREB67
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-4
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-4
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF4
• UniProt Synonym Gene Names: CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67

NCBI Description

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Uniprot Description

Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088).