CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (AP)
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (Biotin)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (MaxLight 405)
Western Blot (WB) (Western Blot analysis of APOBEC2 expression in transfected 293T cell line by APOBEC2 monoclonal antibody (M01), clone 4D7.Lane 1: APOBEC2 transfected lysate (Predicted MW: 25.7 KDa).Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged APOBEC2 is 3 ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) usingMBS6010869. This demonstrates MBS6010869 detected the APOBEC3A protein (arrow).)
Western Blot (WB) (APOBEC4 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the APOBEC4 antibody detected the APOBEC4 protein (arrow).)
Western Blot (WB) (Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) usingMBS6010869. This demonstrates MBS6010869 detected the APOBEC3A protein (arrow).)
Western Blot (WB) (Western Blot analysis of CEM15 in A375 cell lysate, usingMBS629743.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue usingMBS629743, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629743 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (APOBEC3G Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the APOBEC3G antibody detected the APOBEC3G protein (arrow).)
Western Blot (WB) (APOBEC3G Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the APOBEC3G antibody detected the APOBEC3G protein (arrow).)
Western Blot (WB) (Western Blot analysis of CEM15 in A375 cell lysate, usingMBS629743.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue usingMBS629743, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629743 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (Western blot analysis of hPHO3-D314 in mouse testis tissue lysates (35ug/lane). PHO3 (arrow) was detected using the purified Pab.)
Western Blot (WB) (The anti-CEM15 Pab is used in Western blot to detect CEM15 in A549 cell lysate.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (Western Blot detection against Immunogen (36.23kD).)
Western Blot (WB) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody. Lane 1: CDADC1 transfected lysate (58.5kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)
Testing Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Western Blot (WB) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)
Western Blot (WB) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
Western Blot (WB) (The anti-CEM15 Pab is used in Western blot to detect CEM15 in A549 cell lysate.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (Western blot analysis of hPHO3-D314 in mouse testis tissue lysates (35ug/lane). PHO3 (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western Blot detection against Immunogen (36.23kD).)
Western Blot (WB) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody. Lane 1: CDADC1 transfected lysate (58.5kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)
Testing Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Western Blot (WB) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)
Western Blot (WB) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
Western Blot (WB) (The anti-Apobec1 N-term Pab is used in Western blot to detect Apobec in mouse small intestine tissue lysate.)
Western Blot (WB) (Western blot analysis of anti-Apobec1 Antibody (N-term) in 293 cell line lysates (35ug/lane). Apobec1(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS643632. APOBEC3F (arrow) was detected usingMBS643632.)
Immunohistochemistry (IHC) (Immmunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS643632, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS643632 for immunohistochemistry.)
Flow Cytometry (FC/FACS) (Flow Cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) usingMBS643632. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS643632. APOBEC3F (arrow) was detected usingMBS643632.)
Immunohistochemistry (IHC) (Immmunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS643632, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS643632 for immunohistochemistry.)
Flow Cytometry (FC/FACS) (Flow Cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) usingMBS643632. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using Histone H2A.X Antibody.Lane 1: Rat liver, blocked with antigen-specific peptides,Lane 2: Rat liver,Lane 3: Hela cells(serum starvation treatment),Lane 4: HepG2 cells(heat shock treatment).)
Immunofluorescence (IF)/Immunocytochemistry (ICC) (Staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF6823) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Testing Data (ApoBEC3G antibody used at 2.5ug/ml to detect target protein.)
Western Blot (WB) (Western Blot analysis of CDA expression in transfected 293T cell line by CDA polyclonal antibody. Lane 1: CDA transfected lysate (16.2kD). Lane 2: Non-transfected lysate.)
Testing Data (ApoBEC3G antibody used at 2.5ug/ml to detect target protein.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). CDA (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (C-term) in Hela cell line lysates (35ug/lane). CDA (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (Center) in Hela cell line lysates (35ug/lane). CDA(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (Center) in mouse kidney tissue lysates (35ug/lane). CDA(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (Center) in Hela cell line lysates (35ug/lane). CDA(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (Center) in mouse kidney tissue lysates (35ug/lane). CDA(arrow) was detected using the purified Pab.)
Western Blot (WB) (Western Blot analysis of APOBEC3G expression in transfected 293T cell line by APOBEC3G polyclonal antibody. Lane 1: APOBEC3G transfected lysate (46.4kD). Lane 2: Non-transfected lysate.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). CDA (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of anti-CDA Antibody (C-term) in Hela cell line lysates (35ug/lane). CDA (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) usingMBS6010869. This demonstrates MBS6010869 detected the APOBEC3A protein (arrow).)
Western Blot (WB) (APOBEC4 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the APOBEC4 antibody detected the APOBEC4 protein (arrow).)
Western Blot (WB) (CDADC1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CDADC1 antibody detected the CDADC1 protein (arrow).)
Western Blot (WB) (Western blot analysis in ZR-75-1 cell line lysates (35ug/lane) usingMBS6010869. This demonstrates MBS6010869 detected the APOBEC3A protein (arrow).)
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