Rabbit anti-Human APOBEC3G Polyclonal Antibody | anti-APOBEC3G antibody

APOBEC3G, CT (DNA dC->dU-editing Enzyme APOBEC-3G, APOBEC-related Cytidine Deaminase, ARCD, APOBEC-related Protein, ARP-9, APOBEC-related Protein 9, CEM15, CEM-15, MDS019) (Biotin)

Cat. Num. AAA6463535

• Gene Names: APOBEC3G; A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: APOBEC3G; Polyclonal Antibody; CT (DNA dC->dU-editing Enzyme APOBEC-3G; APOBEC-related Cytidine Deaminase; ARCD; APOBEC-related Protein; ARP-9; APOBEC-related Protein 9; CEM15; CEM-15; MDS019) (Biotin); anti-APOBEC3G antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CEM15.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 384

• Applicable Applications for anti-APOBEC3G antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 352-384 in the C-terminal region of human CEM15.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CEM15 in A375 cell lysate, usingMBS629743.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue usingMBS629743, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS629743 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-APOBEC3G antibody

Antibodies; Enzymes; Cytidine Deaminase (APOBEC)

References

1. Kao, S., et al., J. Virol. 77(21):11398-11407 (2003). 2. Stopak, K., et al., Mol. Cell 12(3):591-601 (2003). 3. Mangeat, B., et al., Nature 424(6944):99-103 (2003). 4. Zhang, H., et al., Nature 424(6944):94-98 (2003). 5. Wedekind, J.E., et al., Trends Genet. 19(4):207-216 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 10197640
• NCBI GeneID: 60489
• UniProt Accession #: Q9HC16
• Molecular Weight: 46.408
• NCBI Official Full Name: MDS019
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme catalytic subunit 3G
• NCBI Official Symbol: APOBEC3G
• NCBI Official Synonym Symbols: A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3G
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3G
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3G
• UniProt Synonym Gene Names: APOBEC-related protein; ARCD; ARP-9; CEM15; A3G
• UniProt Entry Name: ABC3G_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene catalyzes site-specific deamination of both RNA and single-stranded DNA. The encoded protein has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Mar 2017]

Uniprot Description

APOBEC3G: DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. May also prevent the transposition of a subset of retroelements. Binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; EC 3.5.4.-; Hydrolase; RNA processing

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; cytosol; ribonucleoprotein complex

Molecular Function: protein binding; protein homodimerization activity; deoxycytidine deaminase activity; zinc ion binding; RNA binding; cytidine deaminase activity

Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; viral reproduction; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus