$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB) (Figure 1. Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb)
Flow Cytometry (FC/FACS) (Figure 3. Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).)
Western Blot (WB) (Figure 1: Western blot analysis using ATF2 mouse mAb against NIH/3T3 cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using ATF2 mouse mAb with DAB staining.)
Testing Data (Figure 1: Western blot analysis using CDK5 mouse mAb against Hela (1), K562 (2), PC-12 (3) and Cos7 (4) cell lysate.)
Testing Data #2 (Figure 2: Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and brain tissues (right) using CDK5 mouse mAb with DAB staining.)
Testing Data #3
Immunohistochemistry (IHC) (Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HCFC1 antibody.)
Western Blot (WB) (Figure 1. Western blot analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.)
Immunofluorescence (IF) (Figure 3. Confocal immunofluorescence analysis of Eca-109 cells using ERK2 mouse mAb (green).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Phospho-Ser166) antibody. Western blot analysis of extracts from COS7 cells, using MDM2 (Phospho-Ser166) antibody.)
Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) antibody.)
Western Blot (WB) (Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: IKBKB antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 87, 86, 30, 80 KDaObserved band size: 87 KDa)
Immunohistochemistry (IHC) (IHC image of MBS7103881 diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (Immunofluorescent analysis of A549 cells using MBS7103881 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of anti-MKK3 Pab in Jurkat cell lysate. MKK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC) (IHC of matched glioma core (top row) and rim (middle row) samples as well as normal brain control (bottom row) from a glioma invasion specific tissue microarray. Insets, individual tumor cells (black squares) are presented at higher magnification to facilitate interpretation of the staining pattern. Staining for MKK3 and pMKK3 exhibits strong signal in majority of invasive cells (B) compared with stationary cells from tumor core (A). Normal brain control shows only weak staining in reactive astrocytes (C).)
Western Blot (WB) (The anti-P38 Pab is used in Western blot to detect P38 in Jurkat cell lysate.)
Western Blot (WB) (Western blot analysis of P38 (arrow) using P38 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (Lane 2) (Origene Technologies).)
Flow Cytometry (FC/FACS) (MAPK14 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of MAPK14 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB) (Western blot analysis of extracts of 293T cellline, using PAK1antibody. Immunohistochemistryof paraffin-embeddedhuman liver cancer tissue usingPAK1antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded M. testis section using M CCNB1 Antibody (Center S123/S125). MBS9211743 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded M. spleen section using M CCNB1 Antibody (Center S123/S125). MBS9211743 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Western Blot (WB) (Mouse CCNB1 Antibody (Center S123/S125) western blot analysis in mouse NIH/3T3 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow).)
Western Blot (WB) (Figure 1. Western blot analysis using NGFR mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.)
Immunofluorescence (IF) (Figure 2.Immunofluorescence analysis of EC cells using NGFR mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin. Blue. DRAQ5 fluorescent DNA dye.)
Flow Cytometry (FC/FACS) (Figure 3. Flow cytometric analysis of EC cells using NGFR mouse mAb (green) and negative control (purple).)
Western Blot (WB) (MAPK1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK1 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (MAPK1 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (AKT1 (Thr308) Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow).)
Immunohistochemistry (IHC) (AKT1 antibody (Thr308) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the AKT1 antibody (Thr308) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (AKT1 Antibody (Thr308) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, Rat brain tissueAll lanes: LIFR antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 124 KDaObserved band size: 124 KDa)
Immunohistochemistry (IHC) (IHC image of MBS7107951 diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (IHC image of MBS7107951 diluted at 1:600 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF) (Immunofluorescence staining of Hela cells with MBS7107951 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC) (Peptide: - / + : Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (Ab-1619) antibody.)
Western Blot (WB) (Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using POLR2A (Ab-1619) antibody.)
Immunofluorescence (IF) (PMA: + / - : Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (Ab-1619) antibody.)
Western Blot (WB) (Western blot analysis of IL17RB Antibody (Center) in MDA-MB468 cell line lysates (35ug/lane). IL17RB (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human colon carcinoma reacted with IL17RB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of HepG2 cells using IL17RB Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Figure 1. Western blot analysis using human IgG (Fc specific) mouse mAb against different fusion proteins with human IgG(Fc specific) tag. Figure 1.)
Immunofluorescence (IF) (Figure 2. Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using human IgGFc mouse mAb (green). Blue. DRAQ5 fluorescent DNA dye.)
Western Blot (WB) (Figure 1. Western blot analysis using GSTP1 mouse mAb against PC3 cell lysate (1) and human cerebellum tissue lysate (2).)
Western Blot (WB) (Figure 2. Western blot analysis using GSTP1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GSTP1 cDNA (2).)
Immunohistochemistry (IHC) (Figure 3. Immunohistochemical analysis of paraffin-embedded human prostate tissues using GSTP1 mouse mAb with DAB staining)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using TRA-2alpha antibody. Western blot analysis of extracts from HUVEC cells, using TRA-2alpha antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HeLa cells, using TRA-2alpha antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FRK antibody. Western blot analysis of extracts from Jurkat cells, using FRK antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HUVEC cells, using FRK antibody.)
Western Blot (WB) (Figure 1: Western blot analysis using GABPA mouse mAb against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.)
Western Blot (WB) (Figure 2: Western blot analysis using GABPA mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).)
Immunofluorescence (IF) (Figure 3: Confocal immunofluorescence analysis of Hela cells using GABPA mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
Western Blot (WB) (Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.)
Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-AKT2 mAb)
Immunofluorescence (IF) (Figure 3: Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Western Blot (WB) (Figure 1: Western blot analysis using CHUK mouse mAb against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.)
Immunofluorescence (IF) (Figure 2: Immunofluorescence analysis of NIH/3T3 cells using CHUK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Flow Cytometry (FC/FACS) (Figure 3: Flow cytometric analysis of A549 cells using CHUK mouse mAb (blue) and negative control (red).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using GAB1 (Phospho-Tyr659) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using GAB1 (Phospho-Tyr659) antibody.)
Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HepG2 cells, using GAB1 (Phospho-Tyr659) antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM (Phospho-Thr199) antibody. Western blot analysis of extracts from Jurkat cells, using NPM (Phospho-Thr199) antibody.)
Immunofluorescence (IF) (EGF +- Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PKC-pan (Ab-497) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins), using PKC-pan (Ab-497) antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HuvEc cells, using PKC-pan (Ab-497) antibody.)
Testing Data (Immunocytochemistry stain of Hela using TORC1 antibody (1:300).)
Testing Data #2 (Western blot detection of TORC1(N-terminus) antibody in Hela&Jurkat lysates using TORC1(N-terminus) antibody (1:1000 diluted). Predicted band size: 69KDa Observed band size: 69KDa.)
Testing Data #3 (Immunoprecipitation analysis of Hela cell lysate using CRTC1 antibody.)
Testing Data #4 (Flow Cytometry analysis of K562 cells stained with TORC1(N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.)
Western Blot (WB) (Figure 1: Western blot analysis using RAG2 mouse mAb against RAG2-hIgGFc transfected HEK293 (1)cell lysate.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using APOL4 antibody. Western blot analysis of extracts from LOVO cells and HT-29cells, using APOL4 antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of MCF-7 cells, using APOL4 antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using CALR antibody. Western blot analysis of extracts from COS-7 cells, using CALR antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using CALR antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using JIP1 (Ab-103) antibody. Western blot analysis of extracts from HUVEC cells, using JIP1 (Ab-103) antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of 3T3 cells, using JIP1 (Ab-103) antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Syntaxin 1A (Ab-14) antibody. Western blot analysis of extracts from COS7 cells, using Syntaxin 1A (Ab-14) antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using Syntaxin 1A (Ab-14) antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Calnexin (Ab-583) antibody. Western blot analysis of extracts from HeLa cells, treated with EGF (200ng/ml, 30mins), using Calnexin (Ab-583) antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HeLa cells, using Calnexin (Ab-583) antibody.)
Testing Data (Figure 1. Western blot analysis using MYST1 mouse mAb against Hela (1), HepG2 (2) and SMMC-7721 (3) cell lysate.)
Testing Data #2 (Figure 3. Confocal immunofluorescence analysis of Eca 109 cells using MOF/MYST1 mouse mAb (green), showing nuclear localization.)
Testing Data #3 (Figure 2. Immunohistochemical analysis of paraffin-embedded human esophageal squamous cell carcinoma (A), normal esophagus epithelium (B), rectum adenocarcinoma (C), lung squamous cell carcinoma (D), breast infiltrating carcinoma (E), and breast infiltrating carcinoma (F) tissues, showing nuclear localization using MOF/MYST1 mouse mAb with DAB staining.)
Western Blot (WB) (Figure 1. Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.)
Immunofluorescence (IF) (Figure 3. Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using CDC25B (Ab-353) antibody. Western blot analysis of extracts from Raw264.7 cells, treated with UV (15mins), using CDC25B (Ab-353) antibody.)
Immunofluorescence (IF) (Peptide-+ Immunofluorescence analysis of HeLa cells, using CDC25B (Ab-353) antibody.)