Testing Data
(P-peptide-+)
NPM Antibody | anti-NPM antibody
NPM (Phospho-Thr199) Antibody
IF: 1:100~1:500
IHC: 1:50~1:100
ELISA: 1:60000
Mouse: Thr198
Rat: Thr198
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM (Phospho-Thr199) antibody. Western blot analysis of extracts from Jurkat cells, using NPM (Phospho-Thr199) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NPM (Phospho-Thr199) antibody. Western blot analysis of extracts from Jurkat cells, using NPM (Phospho-Thr199) antibody.)
Immunofluorescence (IF)
(EGF +- Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.)
Immunofluorescence (IF)
(EGF +- Immunofluorescence analysis of HeLa cells, treated with EGF (200nM, 5mins), using NPM (Phospho-Thr199) antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
Uniprot Description
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Research Articles on NPM
Similar Products
Product Notes
The NPM npm1 (Catalog #AAA852541) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NPM (Phospho-Thr199) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 IHC: 1:50~1:100 ELISA: 1:60000. Researchers should empirically determine the suitability of the NPM npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPM, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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