Western Blot (WB)
(Figure 1. Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.)
Mouse MSH2 Antibody | anti-MSH2 antibody
MSH2 Antibody
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
MSH2; MSH2 Antibody; FCC1; COCA1; HNPCC; LCFS2; anti-MSH2 antibody
Host
Mouse
Reactivity
Human, Monkey
Isotype
IgG1
Clone Number
1B3=3A2B8C
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
197
Applicable Applications for anti-MSH2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of human MSH2 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C
Western Blot (WB)
(Figure 1. Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.)
Western Blot (WB)
(Figure 1. Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.)
Immunofluorescence (IF)
(Figure 3. Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin.)
Immunofluorescence (IF)
(Figure 3. Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin.)
Related Product Information for anti-MSH2 antibody
MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
Product Categories/Family for anti-MSH2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
105kDa
NCBI Official Full Name
induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
NCBI Official Synonym Full Names
MCL1, BCL2 family apoptosis regulator
NCBI Official Symbol
MCL1
NCBI Official Synonym Symbols
TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1
Protein Family
UniProt Gene Name
MCL1
UniProt Synonym Gene Names
BCL2L3; Bcl2-L-3
NCBI Description
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
Uniprot Description
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Research Articles on MSH2
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Product Notes
The MSH2 mcl1 (Catalog #AAA850999) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MSH2 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MSH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the MSH2 mcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSH2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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