CXCL12 (SDF-1 beta) Recombinant Protein | CXCL12 recombinant protein

CXCL12 (SDF-1 beta)

Cat. Num. AAA517610

• Gene Names: CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• Applications: Cell Assay
• Synonyms: CXCL12 (SDF-1 beta); Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; Pre-B cell growth-stimulating factor; PBSF. Recombinant Human Stromal Cell-Derived Factor-1 Beta (CXCL12).; CXCL12 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in sodium phosphate and NaCl (pH7.5)

• Applicable Applications for CXCL12 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Sample Cell Assay Data: Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 5.0-40ng/mL.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human CXCL12 (SDF-1 beta) resolved on a 17% SDS-PAGE gel under non-reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for CXCL12 recombinant protein

Recombinant Human CXCL12 (SDF-1 beta) was expressed in E Coli cells. The protein accession number is P48061
A member of the CXC chemokine family, CXCL12 is strongly chemotactic for T-, B-, and cancer cells. Known to exert its effects mainly through binding to the CXCR4 receptor, CXCL12 plays an important role in morphogenesis, angiogenesis, and cancer metastasis. CXCL12 also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12. Encoded by a single gene, SDF-1 alpha (89 amino acids) and SDF-1 beta (93 amino acids) are derivatives of an alternative splicing event. SDF-1alpha and SDF-1beta have identical amino acid sequences, differing only, by the presence of four extra amino acids at the C-terminus of the beta form. SDF-1 beta is constitutively expressed by stromal cells.

Product Categories/Family for CXCL12 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 76563933
• NCBI GeneID: 6387
• NCBI Accession #: NP_001029058.1
• NCBI GenBank Nucleotide #: NM_001033886.2
• UniProt Accession #: P48061; Q2L985; Q2L986; Q2L988; Q5IT36; Q6ICW0; Q9H554; B2R4G0; E7EVL0; H7BYN8
• Molecular Weight: 10,666 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform gamma
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: CXCL12
• NCBI Official Synonym Symbols: IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• NCBI Protein Information: stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
• UniProt Protein Name: Stromal cell-derived factor 1
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: CXCL12
• UniProt Synonym Gene Names: SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
• UniProt Entry Name: SDF1_HUMAN

NCBI Description

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.1

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; receptor binding

Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; adult locomotory behavior; blood circulation; neuron migration; motor axon guidance; chemotaxis; signal transduction; induction of positive chemotaxis; germ cell development; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; patterning of blood vessels; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; response to mechanical stimulus; response to heat; ameboidal cell migration; response to hypoxia; positive regulation of axon extension involved in axon guidance; immune response; positive regulation of endothelial cell proliferation; telencephalon cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To