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109 results for "dna-damage-response-protein-kinase" - showing 1-50
Testing Data
(ATM-mediated two-step response to DNA double strand breaks.)
ATM, Polyclonal Antibody (Cat# AAA5309729)
Full Name
ATM antibody
Gene Names
ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Applications
IF, WB
Purity
Purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
DNA-PKcs, Monoclonal Antibody (Cat# AAA475013)
Full Name
Anti-DNA-PKcs Mouse mAb
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
Reactivity
Human
Applications
WB, IP, ICC, IHC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot detection of DNA-PKcs in Hela, K562, HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa, Observed band size:450KDa.)
DNA-PKcs, Monoclonal Antibody (Cat# AAA475066)
Full Name
Anti-DNA-PKcs Mouse mAb
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
Reactivity
Human
Applications
WB, IP, ICC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Published customer image: Confirmation and in vitro characterization of Hit compounds. A) HeLa cells were treated with the ten compounds that produced the strongest reduction in pSer40/41MCM2 levels in the primary screen. Protein extracts were prepared and levels of residual pSer40/41MCM2 phosphorylation were measured by western blot analysis with the indicated antibodies. B) The same compounds were tested for their ability to inhibit CDC7 kinase activity in in vitro kinase assay. Kinase reactions were performed on a synthetic MCM2 substrate in the absence or presence of the indicated drug and reactions were run on SDS-PAGE gels. CDC7 activity on the synthetic MCM2 substrate was monitored by Western blotting with an anti-pSer108MCM2 antibody. As a control the levels of CDC7 kinase and synthetic MCM2 substrate present in each reaction were also assessed and shown to be similar in all reactions.From: FitzGerald J, Murillo LS, O'Brien G, O'Connell E, O'Connor A, et al. (2014) A High Through-Put Screen for Small Molecules Modulating MCM2 Phosphorylation Identifies Ryuvidine as an Inducer of the DNA Damage Response. PLoS ONE 9(6): e98891.)
MCM2, Monoclonal Antibody (Cat# AAA212357)
Full Name
MOUSE ANTI HUMAN MCM2
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Applications
WB
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Western Blot (WB)
(Western blot detection of Ku80 in COS7, Jurkat, Hela, MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.)
Ku80, Monoclonal Antibody (Cat# AAA475194)
Full Name
Anti-Ku80 Mouse mAb
Gene Names
XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Reactivity
Human, Monkey
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot detection of Ku80 in A549, MCF7 and Hela cell lysates using Ku80 mouse mAb (dilution 1:500).Predicted band size:86KDa.Observed band size:86KDa.)
Ku80, Monoclonal Antibody (Cat# AAA475619)
Full Name
Anti-Ku80 Mouse mAb
Gene Names
XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Reactivity
Human
Applications
WB, ICC, IP
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
CHEK2, Monoclonal Antibody (Cat# AAA831440)
Full Name
CHEK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
FC/FACS, IF, IHC, WB
Purity
CHEK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Testing Data (TD)
(MBS9203594 staining CDKN1A in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody)
CDKN1A, Polyclonal Antibody (Cat# AAA9203594)
Full Name
CDKN1A Antibody (C-term)
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human, mouse
Applications
IHC-P , WB , IF , EIA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF)
(MBS9604827 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
BRSK1, Polyclonal Antibody (Cat# AAA9604827)
Full Name
BRSK1 Antibody
Gene Names
BRSK1; hSAD1
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
CHEK2, Monoclonal Antibody (Cat# AAA533282)
Full Name
CHEK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
FC/FACS, IF, IHC, WB
Purity
CHEK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunoflurescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(BF8013) and rabbit anti-beta tubulin Ab(AF7011) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
p53, Monoclonal Antibody (Cat# AAA9629032)
Full Name
p53 Mouse monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse
Applications
WB, IF, ICC
Purity
Affinity-chromatography.
Pricing
$280/0.1 mL | $325/0.2 mL | $1,305/5x0.2 mL
Immunohistochemistry (IHC)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.)
p38 MAPK, Monoclonal Antibody (Cat# AAA9629034)
Full Name
p38 MAPK Monoclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-chromatography.
Pricing
$280/0.1 mL | $325/0.2 mL | $1,305/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
c ABL (ABL1), Polyclonal Antibody (Cat# AAA9213319)
Full Name
c ABL (ABL1) Antibody (C-term)
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
Reactivity
Human
Applications
EIA, IHC-P, WB
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
CHEK2, Monoclonal Antibody (Cat# AAA830614)
Full Name
CHEK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
FC/FACS, IHC, WB
Purity
CHEK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
CHEK2, Monoclonal Antibody (Cat# AAA830483)
Full Name
CHEK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
FC/FACS, IHC, WB
Purity
CHEK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
CHEK2, Monoclonal Antibody (Cat# AAA831993)
Full Name
CHEK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
FC/FACS, IHC, WB
Purity
CHEK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CHEK2 protein (Right) or empty vector (Left) detected with CHEK2 antibody)
CHEK2, Monoclonal Antibody (Cat# AAA831170)
Full Name
CHEK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
FC/FACS, IHC, WB
Purity
CHEK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB)
(Western Blot showing CHK2 antibody used against cell lysate from various cell types.)
CHK2, Monoclonal Antibody (Cat# AAA830523)
Full Name
CHK2 antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Applications
EIA, IF, IHC, WB
Pricing
$675/0.1 mL | $3,020/5x0.1 mL
RAD9, Polyclonal Antibody (Cat# AAA222775)
Full Name
RABBIT ANTI YEAST RAD9
Applications
EIA, WB
Pricing
$430/0.05 mg | $1,780/5x0.05 mg
Western Blot (WB)
GADD34, Polyclonal Antibody (Cat# AAA540463)
Full Name
GADD34 Antibody
Gene Names
Gadd34; CG3825; dGADD34; Dm-GADD34; DmelCG3825; dPPP1R15; gadd34; GADD34
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Monkey
Predicted: Bovine, Monkey
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
(Western blot detection of XRCC1 in Raji, K562, Hela, Jurkat and THP-1 cell lysates using XRCC1 mouse mAb (1:1000 diluted).Predicted band size:82KDa.Observed band size:82KDa.)
XRCC1, Monoclonal Antibody (Cat# AAA475210)
Full Name
Anti-XRCC1 Mouse mAb
Gene Names
XRCC1; RCC
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
CHK2, Recombinant Protein (Cat# AAA5304615)
Full Name
CHK2 protein
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Purity
CHK2 protein was purified using a a proprietary chromatographic technique.
Pricing
$555/0.005 mg | $2,490/5x0.005 mg
Chk2 (NT), Polyclonal Antibody (Cat# AAA194194)
Full Name
Chk2 (NT) Polyclonal Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425
Applications
Immunoblot
Pricing
$475/0.1 mg | $2,090/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from 293, mouse NIH/3T3, rat C6 cell line and mouse kidney tissue lysate(from left to right), using Mouse Cdk9 Antibody (C-term). MBS9208988 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Cdk9, Polyclonal Antibody (Cat# AAA9208988)
Full Name
Mouse Cdk9 Antibody (C-term)
Gene Names
Cdk9; PITALRE
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-HIPK2-pY361 Antibody (MBS9211178) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Phospho-HIPK2 (Y361), Polyclonal Antibody (Cat# AAA9211178)
Full Name
Phospho-HIPK2 (Y361) Antibody
Gene Names
HIPK2; PRO0593
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
DB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
SESN1, Polyclonal Antibody (Cat# AAA540251)
Full Name
SESN1 Antibody
Gene Names
SESN1; PA26; SEST1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
PLK1, Polyclonal Antibody (Cat# AAA835357)
Full Name
PLK1 antibody (Ser482,486,490)
Applications
WB
Purity
PLK1 antibody was purified by affinity chromatography
Pricing
$640/0.1 mL | $2,870/5x0.1 mL
Western Blot (WB)
(The anti-ARK5 Pab is used in Western blot to detect ARK5 in mouse brain tissue lysate.)
ARK5, Polyclonal Antibody (Cat# AAA9202740)
Full Name
ARK5 Antibody (N-term)
Gene Names
NUAK1; ARK5
Reactivity
Human, Hamster, Mouse
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
DNA damage response protein kinase DUN1 (DUN1), Recombinant Protein (Cat# AAA1138556)
Full Name
Recombinant Saccharomyces cerevisiae DNA damage response protein kinase DUN1 (DUN1) , partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Western Blot (WB)
(Mouse Nek4 Antibody (N-term) (MBS9212347) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Nek4 antibody detected the Nek4 protein (arrow).)
Nek4, Polyclonal Antibody (Cat# AAA9212347)
Full Name
Mouse Nek4 Antibody (N-term)
Gene Names
Nek4; Stk2
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of anti-CK1d C-term Pab (MBS9207271) in HeLa cell lysate. CK1d (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
CSNK1D, Polyclonal Antibody (Cat# AAA9207271)
Full Name
CSNK1D Antibody (C-term)
Gene Names
CSNK1D; ASPS; HCKID; FASPS2; CKIdelta
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
CHK1, Recombinant Protein (Cat# AAA5304591)
Full Name
CHK1 protein
Gene Names
CHEK1; CHK1
Purity
CHK1 protein was purified using a a proprietary chromatographic technique.
Pricing
$555/0.005 mg | $2,490/5x0.005 mg
SESN1, Polyclonal Antibody (Cat# AAA544836)
Full Name
SESN1 Antibody BIOTIN-Conjugated
Gene Names
SESN1; PA26; SEST1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
SESN1, Polyclonal Antibody (Cat# AAA544837)
Full Name
SESN1 Antibody FITC-Conjugated
Gene Names
SESN1; PA26; SEST1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
SAD1 (BRSK1), Blocking Peptide (Cat# AAA9227609)
Full Name
SAD1 (BRSK1) Antibody (N-term) Blocking peptide
Gene Names
BRSK1; hSAD1
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin G antibody)
Cyclin G, Polyclonal Antibody (Cat# AAA5302486)
Full Name
Cyclin G antibody
Gene Names
CCNG1; CCNG
Applications
WB, IHC, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
BRSK1, Blocking Peptide (Cat# AAA9227610)
Full Name
BRSK1 Antibody (C-term) Blocking Peptide
Gene Names
BRSK1; hSAD1
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-DNA PKcs (S2056) Antibody(MBS1750238)PRKDC was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRKDC Antibody (MBS1750238)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
DNA PKcs, Monoclonal Antibody (Cat# AAA1750238)
Full Name
Anti-Phospho-DNA PKcs (S2056) Rabbit Monoclonal Antibody
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
Reactivity
Human
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
SESN1, Positive Control (Cat# AAA543239)
Full Name
SESN1 Antibody Western Blot Positive Control
Gene Names
SESN1; PA26; SEST1
Applications
WB
Pricing
$335/5 Applications | $1,350/5x5 Applications
IKBKE, Polyclonal Antibody (Cat# AAA1488780)
Full Name
Rabbit Anti Human IKBKE PolyClonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$325/0.1 mL | $1,465/5x0.1 mL
BRSK1, Blocking Peptide (Cat# AAA9228621)
Full Name
BRSK1 Blocking Peptide(Center)
Gene Names
Brsk1; Sad-b; RGD1563268
SESN1, Blocking Peptide (Cat# AAA544450)
Full Name
SESN1 Antibody Antigenic Blocking Peptide
Gene Names
SESN1; PA26; SEST1
Applications
Immunodepletion, Immunocompetition
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver, using Phospho-ATM (S1981) Antibody(MBS1750180)ATM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ATM Antibody (MBS1750180)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
ATM, Monoclonal Antibody (Cat# AAA1750180)
Full Name
Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody
Gene Names
ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Reactivity
Human, Mouse
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate (MBS179540).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK1 )
Chk1, Monoclonal Antibody (Cat# AAA179540)
Full Name
Anti-Chk1 Rabbit Monoclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Csnk1d, Blocking Peptide (Cat# AAA9221026)
Full Name
Mouse Csnk1d Antibody (Center) Blocking Peptide
Gene Names
Csnk1d; AA409348; 1200006A05Rik; D930010H05Rik
Western Blot (WB)
(Western blot analysis of Phospho-Chk1 (S296) expression in HEK293 cell lysate Treated with Calyculin (MBS1750252).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHEK1 )
Chk1, Monoclonal Antibody (Cat# AAA1750252)
Full Name
Anti-Phospho-Chk1 (S296) Rabbit Monoclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
CSNK1D, Blocking Peptide (Cat# AAA9226859)
Full Name
CSNK1D Antibody (C-term) Blocking peptide
Gene Names
CSNK1D; ASPS; HCKID; FASPS2; CKIdelta
Western Blot (WB)
(Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate (MBS1750174).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR, Monoclonal Antibody (Cat# AAA1750174)
Full Name
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
CDK2 / Cyclin-Dependent Kinase 2, Recombinant Protein (Cat# AAA2903049)
Full Name
Human CDK2 / Cyclin-Dependent Kinase 2 Recombinant Protein
Gene Names
CDK2; CDKN2; p33(CDK2)
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(Western blot analysis of DNA-PKcs expression in HeLa cell lysate (MBS179403).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKDC monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKDC )
DNA-PKcs, Monoclonal Antibody (Cat# AAA179403)
Full Name
Anti-DNA-PKcs Rabbit Monoclonal Antibody
Gene Names
PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
