CHK1 recombinant protein

CHK1 protein

Cat. Num. AAA5304591

• Gene Names: CHEK1; CHK1
• Purity: CHK1 protein was purified using a a proprietary chromatographic technique.
• Synonyms: CHK1; CHK1 protein; CHK-1 protein; Serine/threonine-protein kinase Chk1 protein; CHK protein; CHK-1; CHK 1 protein; CHK 1; CHK1 recombinant protein

• Host: Human
• Purity/Purification: CHK1 protein was purified using a a proprietary chromatographic technique.
• Form/Format: Supplied in a storage buffer consisting of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol
• Sequence Length: 476

• Protein Type: Recombinant
• Biological Significance: CHK1 is a 56 kd serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia. In vitro, CHK1 binds to and phosphorylates the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 phosphorylates Cdc25C on serine-216 which creates a binding site for 14-3-3 protein and inhibits function of the phosphatase. Thus, in response to DNA damage, CHK1 phosphorylates and inhibits Cdc25C, thereby preventing the activation of the Cdc2-cyclin B complex and mitotic entry.
• Expression System: Sf-9 insect cells
• Preparation and Storage: Store at -80 degree C

Related Product Information for CHK1 recombinant protein

Purified recombinant Human CHK1 protein

Product Categories/Family for CHK1 recombinant protein

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 2367669
• NCBI GeneID: 1111
• Molecular Weight: 82 kDa
• NCBI Official Full Name: Chk1
• NCBI Official Synonym Full Names: checkpoint kinase 1
• NCBI Official Symbol: CHEK1
• NCBI Official Synonym Symbols: CHK1
• NCBI Protein Information: serine/threonine-protein kinase Chk1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• Protein Family: Histidine protein kinase
• UniProt Gene Name: CHEK1
• UniProt Synonym Gene Names: CHK1
• UniProt Entry Name: CHK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.

Protein type: EC 2.7.11.1; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMKL family; CHK1 subfamily

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: nucleoplasm; centrosome; extracellular space; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; cytosol; nucleus; chromatin

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; DNA repair; DNA replication; G2/M transition DNA damage checkpoint; G2/M transition of mitotic cell cycle; response to DNA damage stimulus; regulation of cell proliferation