Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
anti-Human DNA-PKcs Monoclonal Antibody | anti-DNA-PKcs antibody
Anti-DNA-PKcs Mouse mAb
Source: Human
ICC: 1:100
Other: 1:1000
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).)
Western Blot (WB)
(Western blot detection of DNA-PKcs in Hela, Molt-4, A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
Western Blot (WB)
(Western blot detection of DNA-PKcs in Hela, Molt-4, A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
Uniprot Description
DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: ATYPICAL group; DNA repair, damage; DNAPK subfamily; EC 2.7.11.1; Kinase, protein; Nucleolus; PIKK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical
Chromosomal Location of Human Ortholog: 8q11.21
Cellular Component: cytosol; DNA-dependent protein kinase complex; extracellular matrix; membrane; nuclear chromosome, telomeric region; nucleoplasm; transcription factor complex
Molecular Function: DNA-dependent protein kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; transcription factor binding
Biological Process: cell proliferation; cellular response to insulin stimulus; double-strand break repair; double-strand break repair via nonhomologous end joining; negative regulation of apoptosis; negative regulation of protein amino acid phosphorylation; peptidyl-serine phosphorylation; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; protein modification process; regulation of circadian rhythm; regulation of smooth muscle cell proliferation; telomere capping
Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities
Research Articles on DNA-PKcs
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Product Notes
The DNA-PKcs prkdc (Catalog #AAA475013) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-DNA-PKcs Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA-PKcs can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC). WB: 1:1000 ICC: 1:100 Other: 1:1000. Researchers should empirically determine the suitability of the DNA-PKcs prkdc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA-PKcs, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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