Rabbit RAD9 Polyclonal Antibody | anti-RAD9 antibody

RABBIT ANTI YEAST RAD9

Cat. Num. AAA222775

• Applications: ELISA, Western Blot
• Synonyms: RAD9; Polyclonal Antibody; RABBIT ANTI YEAST RAD9; anti-RAD9 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability. ; ; This antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 1309

• Applicable Applications for anti-RAD9 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 60kDa. ; ELISA: Minimum Dilution: 1/2000; Maximum Dilution: 1/4000; Western Blotting: Minimum Dilution: 1/50; Maximum Dilution: 1/250
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).; Buffer Solution
• Antiserum Preparation: Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Yeast
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-RAD9 antibody

MBS222775 detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability. This antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.

NCBI and Uniprot Product Information

• NCBI GI #: 6320423
• NCBI GeneID: 851803
• NCBI Accession #: NP_010503.1
• NCBI GenBank Nucleotide #: NM_001180525.1
• UniProt Accession #: P14737; Q04920; D6VSK0
• Molecular Weight: 148,398 Da
• NCBI Official Full Name: chromatin-binding protein RAD9
• NCBI Official Symbol: RAD9
• NCBI Protein Information: chromatin-binding protein RAD9
• UniProt Protein Name: DNA repair protein RAD9
• Protein Family: RAD9, HUS1, RAD1-interacting nuclear orphan protein
• UniProt Gene Name: RAD9
• UniProt Entry Name: RAD9_YEAST

Uniprot Description

Essential for cell cycle arrest at the G2 stage following DNA damage by X-irradiation or inactivation of DNA ligase.