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37 results for "Fluorescent Probe" - showing 1-37
Reactive Oxygen Species (ROS) Detection, Assay Kit (Cat# AAA9719224)
Full Name
Reactive Oxygen Species (ROS) Detection Fluorometric Assay Kit
Pricing
$205/100 Tests | $175/50 Tests | $935/5x100 Tests
Testing Data
(Jurkat suspension cells were exposed to 1 uM of staurosporine for 4 hours at 37 degree C to induce apoptosis. Cells were dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red fluorescent vital dye 7-AAD to detect necrosis. The image shown in panel A reveals 4 populations of cells: 1) Live, unstained cells, which do not fluoresce. 2) Early stage apoptotic cells fluoresce green with FAM-FLICA. 3) Dually stained green and red fluorescing cells represent the population of Jurkat cells in mid-to-late stage apoptosis; these cells have active caspase enzymes and compromised cell membranes. 4) Necrotic cells fluoresce red. Panel B shows a corresponding differential interference contrast (DIC) image, which reveals cell morphology.)
Necrosis vs Apoptosis, Assay Kit (Cat# AAA258047)
Full Name
Necrosis vs Apoptosis Assay Kit
Pricing
$670/100-200 Tests | $405/50-100 Tests
Immunofluorescence (IF)
(Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
DAPI, Reagent (Cat# AAA826728)
Full Name
DAPI Staining Kit
Applications
IF
Pricing
$130/0.1 mL | $540/5x0.1 mL
Testing Data
(Jurkat cells were treatedwith staurosporine, anapoptosis-inducingagent (bottom), orDMSO, a negativecontrol (top), for 4hours, then incubatedwith ICT's green caspase6 inhibitor probe,FAM-VEID-FMK, for 1hour. Cells were washedtwice and read on a flowcytometer. Treatment with staurosporine induced caspase 6 activity in 95.2% ofthe experimental cells (P3, bottom right), whereas the negative controltreatment exhibited caspase 6 activity in only 5.9% of the cell population (P3,top right). This is a ratio of 16:1. (Dr. Brian W. Lee, ICT).)
FAM-FLICA Caspase 6, Assay Kit (Cat# AAA258019)
Full Name
FAM-FLICA Caspase 6 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Immunofluorescence (IF)
(Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).)
Hoechst 33342, Reagent (Cat# AAA826727)
Full Name
Hoechst 33342 Staining Kit
Applications
IF
Pricing
$130/0.1 mL | $540/5x0.1 mL
Testing Data
(Jurkat cells were treated withstaurosporine, an apoptosis-inducingagent (bottom), or DMSO, a negativecontrol (top), for 4 hours, thenincubated with ICT's green caspase 10inhibitor probe, FAM-AEVD-FMK, for 1hour. Cells were washed twice and readon a flow cytometer. Treatment withstaurosporine induced caspase 10activity in 93.6% of the experimentalcells (M2, bottom right), whereas thenegative control treatment exhibitedcaspase 10 activity in only 4.7% of thecell population (M2, top right). This is a ratio of 20:1. (Dr. Brian W. Lee, ICT).)
FAM-FLICA Caspase 10, Assay Kit (Cat# AAA258025)
Full Name
FAM-FLICA Caspase 10 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Testing Data
(Jurkat cells were grown to 5 x 10^5 cells/mL and split into two populations. One population (left) was left untreated (live) while the other population (right) was treated with 90% ethanol for 60 seconds to create a dead cell population. Cells that were exposed to ethanol (right) were treated with a 5-fold larger volume of PBS to stop the ethanol surface denaturation process. Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (left) are dead compared with 97.6% of the treated cells (right) (ICT 226:30-31).)
7-AAD Red Fluorescent Live/Dead Stain, Reagent (Cat# AAA258053)
Full Name
7-AAD Red Fluorescent Live/Dead Stain
Pricing
$245/125 Tests | $1,065/5x125 Tests
Hoechst 33342/PI, Reagent (Cat# AAA826730)
Full Name
Hoechst 33342/PI Double Staining Kit
Applications
IF
Pricing
$150/0.1 mL | $615/5x0.1 mL
c-erbB2 DNA Probe, cDNA Clone (Cat# AAA652225)
Full Name
c-erbB2 DNA Probe (HER2, neu Receptor), BioGenomics
Applications
IHC
Pricing
$810/0.4 mL | $3,630/5x0.4 mL
Testing Data
(Jurkat cells were treatedwith staurosporine, anapoptosis-inducing agent(bottom), or DMSO,a negative control (top), for4 hours, then incubatedwith ICT's green caspase 2 inhibitor probe, FAM-VDVAD-FMK, for 1 hour. Cells were washed twice and read on a flow cytometer. Treatment with staurosporine induced caspase2 activity in 63.2% of the experimental cells (M2, bottom right), whereas thenegative control treatment exhibited caspase 2 activity in only 7.2% of the cellpopulation (M2, top right). This is a ratio of 9:1. (Dr. Brian W. Lee, ICT).)
FAM-FLICA Caspase 2, Assay Kit (Cat# AAA258004)
Full Name
FAM-FLICA Caspase 2 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Testing Data
(Immunofluorescence staining of mouse anterior horns of spinal cord at 6 months, 15 months and 26 months of age. Antibody used at 1:100 dilution and visualized using donkey anti-sheep conjugated to Alexa 555 dye (red color). The blue color is a fluorescent nuclear probe, TOPRO3. (Image modified from original in citation 1))
RED 1, Polyclonal Antibody (Cat# AAA395871)
Full Name
RED 1
Gene Names
Adarb1; Red1; Adar2
Reactivity
Rat
Applications
WB
Purity
Antigen Immunoaffiinity Purification
Pricing
$390/0.05 mg | $1,610/5x0.05 mg
Testing Data
(Mouse EL4 thymoma cellswere cultured in the absence(A) or presence (B) of 100 ugmitomycin for 18 hours. Thecells were harvested andstained with ICT'sSR-DEVD-FMK inhibitor probe(red histograms) to detect active caspases 3&7. The data illustrate a low level ofcaspase-3 activity in untreated cells (A) compared to unstained control (bluehistogram); caspase-3 activity is greatly enhanced in the mitomycin treatedcultures (B). Cells were analyzed with a BD LSRII flow cytometer to detectsulforhodamine using the green laser (532nm) and detector D set up with filter610/620 600LP (Dr. Catherine Martone, Yale University).)
SR FLICA Caspase 3 and 7, Assay Kit (Cat# AAA258021)
Full Name
SR FLICA Caspase 3 and 7 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Testing Data
(Jurkat cells were treated with 1 uM staurosporine toinduce caspase 9 activity (left), or a negative control(right) for 3 hours, washed twice, then incubated withICT's red caspase 9 inhibitor probe, SR-LEHD-FMK, for 1hour and examined under a fluorescence microscope.DIC images were also taken of all cells. Both inducedimages reveal several experimental cells which fluorescered, therefore they have some degree of caspase 9activity. The non-induced DIC image reveals many controlcells, however the corresponding fluorescence image isdark; none of these cells have active caspase 9 (Dr. BrianW. Lee, ICT).)
SR-FLICA Caspase 9, Assay Kit (Cat# AAA258030)
Full Name
SR-FLICA Caspase 9 Assay Kit
Pricing
$665/100 Tests | $300/25 Tests | $3,010/5x100 Tests
Testing Data
(Image: Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (green) and NF-H (red), counterstained with a fluorescent DNA probe (blue).)
GAPDH, Antibody (Cat# AAA555011)
Full Name
Mouse Monoclonal Anti-GAPDH
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Bovine, Chicken, Human, Mouse, Porcine, Rat
Applications
IHC, WB
Pricing
$425/0.1 mL | $1,760/5x0.1 mL
Structure
(Structure of biotinylated EGRCK. The SPACER represent a carbon spacer used to optimize the reactivity of the biotin group after the probe has been reacted with the active site of the serine protease.)
Chloromethylketone EGR, Protein (Cat# AAA634442)
Full Name
Chloromethylketone EGR (EGRCK) (Biotin)
Applications
EL/EIA, WB, IF
Purity
Purified
Determined by HPLC and TLC analyses
Determined by HPLC and TLC analyses
Pricing
$350/0.1 mg | $1,030/1 mg | $4,630/5x1 mg
Chemical Structure
(Chemical structure of JC-1 , a Mitochondrial membrane potential dependent fluorescent dye. CAS #: 3520-43-2. Molecular Formula: C25H27Cl4IN4. Molecular Weight: 652.2 g/mol.)
JC-1, Small Molecule (Cat# AAA808256)
Full Name
JC-1
Purity
>98% (HPLC); NMR (Conforms)
Pricing
$205/1 mg | $320/5 mg | $700/5x1 mg | $1,205/5x5 mg
Structure
Fluorescein Diacetate, Inhibitor (Cat# AAA5756006)
Full Name
Fluorescein Diacetate
Pricing
$195/5 mg | $195/1 mL (in DMSO) | $615/5x5 mg
Glucose Uptake, Assay Kit (Cat# AAA2567973)
Full Name
Glucose Uptake Fluorometric Assay Kit
Pricing
$725/96 Tests
JAK2 JH2 Tracer, Reagent (Cat# AAA3844504)
Full Name
JAK2 JH2 Tracer
Purity
>98%
Pricing
$215/1 mg | $515/5 mg | $850/10 mg | $2,440/50 mg
Structure
Dihydrofluorescein diacetate, Inhibitor (Cat# AAA5755827)
Full Name
Dihydrofluorescein diacetate
Purity
98.00%
Pricing
$205/50 mg | $645/5x50 mg
Hydroxyl Radical Antioxidant Capacity (HORAC), Assay Kit (Cat# AAA9719221)
Full Name
Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit
Pricing
$510/96 Tests | $450/48 Tests | $2,335/5x96 Tests
Oxygen Radical Antioxidant Capacity (ORAC), Assay Kit (Cat# AAA9719220)
Full Name
Oxygen Radical Antioxidant Capacity (ORAC) Fluorometric Assay Kit
Pricing
$510/96 Tests | $450/48 Tests | $2,335/5x96 Tests
Dihydrorhodamine 123, Biochemical (Cat# AAA3844169)
Full Name
Dihydrorhodamine 123
Purity
>98%
Pricing
$125/1 mg | $215/5 mg | $315/10 mg | $850/50 mg | $3,760/5x50 mg
Structure
Structure
Pyranine, Inhibitor (Cat# AAA5755425)
Full Name
Pyranine
Purity
98.00%
Pricing
$195/5 mg | $205/1 mL (in DMSO) | $650/5x1 mL (in DMSO)
Structure
Iron sucrose, Inhibitor (Cat# AAA5758955)
Full Name
Iron sucrose
Purity
98.00%
Pricing
$165/25 mg | $195/100 mg | $205/200 mg | $195/1 mL (in DMSO) | $660/5x200 mg
Chemical Structure
Rhod-2 AM, Accessory Reagent (Cat# AAA3841929)
Full Name
Rhod-2 AM
Purity
>98%
Pricing
$420/1 mg | $720/2 mg | $1,320/5 mg | $5,870/5x5 mg
AbFluor 594-Phalloidin, Cell Staining (Cat# AAA9719186)
Full Name
AbFluor 594-Phalloidin
Pricing
$255/500 Tests | $1,130/5x500 Tests
AbFluor 488-Phalloidin, Cell Staining (Cat# AAA9719184)
Full Name
AbFluor 488-Phalloidin
Pricing
$255/500 Tests | $1,130/5x500 Tests
Real Thiol, Biochemical (Cat# AAA3844105)
Full Name
Real Thiol
Purity
>98%
Pricing
$415/1 mg | $1,140/5 mg | $1,910/10 mg | $5,560/50 mg
Chemical Structure
Zinquin, Accessory Reagent (Cat# AAA3841939)
Full Name
Zinquin
Purity
>98%
Pricing
$140/1 mg | $180/2 mg | $260/5 mg | $420/10 mg | $1,840/5x10 mg
Chemical Structure
TMA-DPH, Accessory Reagent (Cat# AAA3841928)
Full Name
TMA-DPH
Purity
>98%
Pricing
$160/1 mg | $220/2 mg | $375/5 mg | $650/10 mg | $2,870/5x10 mg
IQ-R, Biochemical (Cat# AAA3845310)
Full Name
IQ-R
Purity
>98%
Pricing
$485/5 mg | $805/10 mg | $2,280/50 mg | $4,490/100 mg
Chemical Structure
DDAO, Inhibitor (Cat# AAA5764667)
Full Name
DDAO
Purity
100.0%
Pricing
$200/10 mg | $230/25 mg | $275/50 mg | $355/100 mg | $465/200 mg | $195/1 mL (in DMSO) | $1,820/5x200 mg
AbFluor 555-Phalloidin, Cell Staining (Cat# AAA9719185)
Full Name
AbFluor 555-Phalloidin
Pricing
$255/500 Tests | $1,130/5x500 Tests
