Immunofluorescence (IF)
(Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).)
Hoechst 33342 Reagent
Hoechst 33342 Staining Kit
Applications
Immunofluorescence
Synonyms
Hoechst 33342; Hoechst 33342 Staining Kit; Hoechst 33342 reagent
Applicable Applications for Hoechst 33342 reagent
Immunofluorescence (IF)
Application Notes
Hoechst 33342 is a cell permeable fluorescent minor groove-binding probe for DNA, and specific stain for AT-rich regions of double-stranded DNA. This fluorescent dye has been used in sorting living cells, used in flow cytometry for the determination of DNA and used for visualization of chromatin distribution in living cells. Hoechst 33342 and DNA complex show light blue flourescent color with excitation light 355 nm and emission light 465 nm.
Preparation and Storage
Store at 4 degree C.
Immunofluorescence (IF)
(Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. Hoechst 33342 was used to stain the cell nuclei (blue).)
Related Product Information for Hoechst 33342 reagent
Hoechst 33342 Staining Kit
Similar Products
Product Notes
The Hoechst 33342 (Catalog #AAA826727) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hoechst 33342 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF). Hoechst 33342 is a cell permeable fluorescent minor groove-binding probe for DNA, and specific stain for AT-rich regions of double-stranded DNA. This fluorescent dye has been used in sorting living cells, used in flow cytometry for the determination of DNA and used for visualization of chromatin distribution in living cells. Hoechst 33342 and DNA complex show light blue flourescent color with excitation light 355 nm and emission light 465 nm. Researchers should empirically determine the suitability of the Hoechst 33342 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hoechst 33342, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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