AbFluor 488-Phalloidin Cell Staining
AbFluor 488-Phalloidin
Synonyms
AbFluor 488-Phalloidin; BMD00082; AbFluor 488-Phalloidin cell staining
Form/Format
Lyophilized powder
Tag
F-actin
Features & Benefits
Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Proprietary AbFluor 488-Phalloidin (Green Fluorescence)(Ex/Em = 490/515nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
AbFluor 488-Phalloidin selectively bound to F-actins
it is much higher photostability than the fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels.
Proprietary AbFluor 488-Phalloidin (Green Fluorescence)(Ex/Em = 490/515nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.
AbFluor 488-Phalloidin selectively bound to F-actins
it is much higher photostability than the fluorescein-phalloidin conjugates. Phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, which can stain F-actins in cell cultures or cell-free experiments at nanomolar levels.
Usage Notes
Phalloidin is toxic, it should be handled with care.
Preparation and Storage
Stable for at least 12 months if store at -20 degree C. Protect this product from light.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for AbFluor 488-Phalloidin cell staining
Description: AbFluor 488-Phalloidin selectively bound to F-actins.
Background: Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.
Background: Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.
Product Categories/Family for AbFluor 488-Phalloidin cell staining
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Product Notes
The AbFluor 488-Phalloidin (Catalog #AAA9719184) is a Cell Staining and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AbFluor 488-Phalloidin, Cell Staining" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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