Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
64 results for " actin etc" - showing 1-50
Western Blot (WB)
(Western blot detection of beta actin in Hela, 3T3, C6, CHO-K1 and COS7 cell lysates using beta actin mouse mAb conjugated with HRP (1:10000 diluted), Predicted band size:45KDa.Observed band size:45KDa.)
beta-Actin, Monoclonal Antibody (Cat# AAA475330)
Full Name
Anti-beta-Actin-HRP (HRP-Conjugate) Mouse mAb
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Monkey, Goat, Rat, Hamster, Saccharomyces cerevisiae, Arabidopsis, Pichia pastoris, Rabbit, Zebrafish, Drosophila, Leaf of Rice, Fruit of Cucumber, Chlamydomonas reinhardtii.
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using beta Actin Antibody )
beta Actin, Monoclonal Antibody (Cat# AAA179614)
Full Name
Anti-beta Actin Rabbit Monoclonal Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of FMN1 using anti-FMN1 antibody (MBS1750923).
FMN1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FMN1 Antibody (MBS1750923) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
FMN1, Polyclonal Antibody (Cat# AAA1750923)
Full Name
Anti-FMN1 Picoband Antibody
Gene Names
FMN1; LD; FMN
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
Actin, Smooth Muscle, Antibody (Cat# AAA462003)
Full Name
Rabbit anti SMA antibody (Smooth Muscle Actin)
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, EIA, IP
Purity
The Rabbit IgG is purified by Epitope affinity purification
Pricing
$270/0.1 mg | $1,205/5x0.1 mg
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 2 Antibody at 2 /ml SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 33kDaObserved band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 2, Polyclonal Antibody (Cat# AAA715593)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 2 polyclonal Antibody, Biotin conjugated
Gene Names
ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 2 Antibody at 2 /ml SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 33kDaObserved band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 2, Polyclonal Antibody (Cat# AAA715992)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 2 polyclonal Antibody, HRP conjugated
Gene Names
ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Fig: Western blot analysis on Mouse Heart using anti-a-Actin polyclonal antibody)
Alpha-Actin, Polyclonal Antibody (Cat# AAA857582)
Full Name
Alpha-Actin (Smooth Muscle) Antibody
Gene Names
Actc1; Actc-1
Reactivity
Mouse, Rat, Human
Applications
WB
Purity
Affinity purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 2 Antibody at 2 /ml SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 33kDaObserved band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 2, Polyclonal Antibody (Cat# AAA715953)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 2 polyclonal Antibody, FITC
Gene Names
ARPC2; ARC34; PRO2446; p34-Arc; PNAS-139
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney, Tissue Set (Cat# AAA640973)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 3Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 20 kDaObserved band size: 75 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 3, Polyclonal Antibody (Cat# AAA715585)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 3 polyclonal Antibody, Biotin conjugated
Gene Names
ARPC3; ARC21; p21-Arc
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 3Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 20 kDaObserved band size: 75 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 3, Polyclonal Antibody (Cat# AAA715899)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 3 polyclonal Antibody, FITC
Gene Names
ARPC3; ARC21; p21-Arc
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Lung, Tissue Set (Cat# AAA640990)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Lung
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Western Blot (WB)
(Western blot Actin-related protein 2/3 complex subunit 3Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 20 kDaObserved band size: 75 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Actin-related protein 2/3 complex subunit 3, Polyclonal Antibody (Cat# AAA715765)
Full Name
Rabbit anti-human Actin-related protein 2/3 complex subunit 3 polyclonal Antibody, HRP conjugated
Gene Names
ARPC3; ARC21; p21-Arc
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot detection of beta actin in Jurkat, K562, C6, 3T3 and Hela cell lysates using beta actin mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.)
beta-Actin, Monoclonal Antibody (Cat# AAA475370)
Full Name
Anti-beta-Actin Mouse mAb
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Actin, alpha, Sarcomeric, Monoclonal Antibody (Cat# AAA632860)
Full Name
Anti-Actin, alpha, Sarcomeric (Skeletal, Cardiac Muscle)
Reactivity
Bovine; Frog; Human; Rabbit; Rat; Sheep
Applications
ELISA; Western Blot
Purity
Ascites
Pricing
$785/0.1 mL | $3,525/5x0.1 mL
Western Blot (WB)
(Western blot detection of Phostensin in Hela, Raji, Ramos and Leucocyte cell lysates and using Phostensin mouse mAb (1:200 diluted).Predicted band size: 120KDa.Observed band size: 120KDa.)
Phostensin, Monoclonal Antibody (Cat# AAA475039)
Full Name
Anti-Phostensin Mouse mAb
Gene Names
PPP1R18; HKMT1098; KIAA1949
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Heart, Tissue Set (Cat# AAA641003)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Heart
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Western Blot (WB)
(Western blot analysis of Fibrillarin expression in HepG2 cell lysate (MBS179941).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FBL )
Fibrillarin, Monoclonal Antibody (Cat# AAA179941)
Full Name
Anti-Fibrillarin Rabbit Monoclonal Antibody
Gene Names
FBL; FIB; FLRN; Nop1; RNU3IP1
Reactivity
Human, Mouse, Rat
Applications
IP, Immunofluorescence (IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
beta-Actin, Monoclonal Antibody (Cat# AAA416878)
Full Name
Anti-beta-Actin Antibody
Reactivity
Beta-actin from human, mouse, rat, rabbit or chicken. Beta-actin from other species may also be detectable.
Applications
WB, EIA, DB
Purity
Affinity Purified.
Pricing
$255/0.1 mg | $915/5x0.1 mg
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Small Intestine, Tissue Set (Cat# AAA640959)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Small Intestine
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Western Blot (WB)
Actin-beta, Polyclonal Antibody (Cat# AAA460213)
Full Name
Rabbit anti Actin-beta Polyclonal Antibody
Reactivity
Human,Mouse,Rat,Dog,Bovine,Sheep,Goat
Applications
IHC, WB, IHC
Purity
The Rabbit IgG is purified by EpitopeAffinity Purification.
Pricing
$310/0.1 mg | $1,380/5x0.1 mg
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Skeletal Muscle, Tissue Set (Cat# AAA640983)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Skeletal Muscle
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Liver, Tissue Set (Cat# AAA640981)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Liver
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Skeletal Muscle, Tissue Set (Cat# AAA640974)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Skeletal Muscle
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Lung, Tissue Set (Cat# AAA640977)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Lung
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Heart, Tissue Set (Cat# AAA640988)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Heart
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Brain, Tissue Set (Cat# AAA640997)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Brain
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Brain, Tissue Set (Cat# AAA640986)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Brain
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Liver, Tissue Set (Cat# AAA640996)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Liver
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Stomach, Tissue Set (Cat# AAA640963)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Stomach
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Tissue Set, BioAssay, DNA, RNA, Protein Adult Normal, Kidney, Tissue Set (Cat# AAA640995)
Full Name
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney
Purity
Molecular Biology Grade
Pricing
$605/1 Set | $2,775/5x1 Set
Typical Testing Data/Standard Curve (for reference only)
Dystroglycan, ELISA Kit (Cat# AAA1751136)
Full Name
Human Dystroglycan PicoKine ELISA Kit
Gene Names
DAG1; A3a; DAG; AGRNR; 156DAG; MDDGA9; MDDGC7; MDDGC9
Reactivity
Human
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Applications
EIA
Pricing
$460/96-Strip-Wells | $2,060/5x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of Flotillin 1 expression in HeLa cell lysate (MBS1750019).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FLOT1 )
Flotillin 1, Monoclonal Antibody (Cat# AAA1750019)
Full Name
Anti-Flotillin 1 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate (MBS179786).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTA1 )
Actin, Monoclonal Antibody (Cat# AAA179786)
Full Name
Anti-Actin Rabbit Monoclonal Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Actin, Polyclonal Antibody (Cat# AAA460195)
Full Name
Rabbit anti Actin, skeletal muscle Polyclonal Antibody
Reactivity
Human, Rat, Rabbit, Guinea Pig
Applications
EIA, FC/FACS, IHC, IP, WB
Purity
Purified by Epitope Affinity Purification
Pricing
$270/0.1 mg | $1,205/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody(MBS179787)ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (MBS179787)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Actin, Monoclonal Antibody (Cat# AAA179787)
Full Name
Anti-Actin (Alpha-Actin) Rabbit Monoclonal Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using p16 ARC Antibody(MBS179795)ARPC5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARPC5 Antibody (MBS179795)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
p16 ARC, Monoclonal Antibody (Cat# AAA179795)
Full Name
Anti-p16 ARC Rabbit Monoclonal Antibody
Gene Names
ARPC5; ARC16; p16-Arc; dJ127C7.3
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Actin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate (MBS179785).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTA1 )
Actin, Monoclonal Antibody (Cat# AAA179785)
Full Name
Anti-Actin Rabbit Monoclonal Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using alpha smooth muscle Actin Antibody(MBS179545)ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (MBS179545)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
alpha smooth muscle Actin, Monoclonal Antibody (Cat# AAA179545)
Full Name
Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cortactin expression in HeLa cell lysate (MBS179610).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTTN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTTN )
Cortactin, Monoclonal Antibody (Cat# AAA179610)
Full Name
Anti-Cortactin Rabbit Monoclonal Antibody
Gene Names
CTTN; EMS1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody(MBS179784)ACTA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA1 Antibody (MBS179784)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Actin, Monoclonal Antibody (Cat# AAA179784)
Full Name
Anti-Actin Rabbit Monoclonal Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Ezrin expression in HeLa cell lysate (MBS179733).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EZR )
Ezrin, Monoclonal Antibody (Cat# AAA179733)
Full Name
Anti-Ezrin Rabbit Monoclonal Antibody
Gene Names
EZR; CVL; CVIL; VIL2; HEL-S-105
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PDPK1 expression in HEK293 cell lysate (MBS179572).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDPK1 )
PDPK1/Pdk 1, Monoclonal Antibody (Cat# AAA179572)
Full Name
Anti-PDPK1/Pdk 1 Rabbit Monoclonal Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Troponin T expression in human fetal heart lysate (MBS179571).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNT2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNNT2 )
Troponin T, Monoclonal Antibody (Cat# AAA179571)
Full Name
Anti-Troponin T Rabbit Monoclonal Antibody
Gene Names
TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody(MBS179543)ACTA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACTA2 Antibody (MBS179543)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
alpha smooth muscle Actin, Monoclonal Antibody (Cat# AAA179543)
Full Name
Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of delta1 Catenin expression in HeLa cell lysate (MBS179829).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNND1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNND1 )
delta 1 Catenin/p120 Catenin, Monoclonal Antibody (Cat# AAA179829)
Full Name
Anti-delta 1 Catenin/p120 Catenin Rabbit Monoclonal Antibody
Gene Names
CTNND1; CAS; p120; BCDS2; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rho A expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179185).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RHOA )
Rho A, Monoclonal Antibody (Cat# AAA179185)
Full Name
Anti-Rho A Rabbit Monoclonal Antibody
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Vinculin expression in (1) HepG2 cell lysate; (2) HeLa cell lysate (MBS179590).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VCL )
Vinculin, Monoclonal Antibody (Cat# AAA179590)
Full Name
Anti-Vinculin Rabbit Monoclonal Antibody
Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of alpha smooth muscle Actin expression in A431 cell lysate (MBS179544).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACTA2 )
alpha smooth muscle Actin, Monoclonal Antibody (Cat# AAA179544)
Full Name
Anti-alpha smooth muscle Actin Rabbit Monoclonal Antibody
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ROCK1 expression in Ramos cell lysate (MBS179425).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ROCK1 )
ROCK1, Monoclonal Antibody (Cat# AAA179425)
Full Name
Anti-ROCK1 Rabbit Monoclonal Antibody
Gene Names
ROCK1; ROCK-I; P160ROCK
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
