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19 results for " Vitamins minerals" - showing 1-19
Western Blot (WB)
(Figure 1. Western blot analysis of RBP4 using anti-RBP4 antibody (MBS1750562).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysates,Lane 2: rat liver tissue lysates,Lane 3: human placenta tissue lysates,Lane 4: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBP4 at approximately 23KD. The expected band size for RBP4 is at 23KD. )
RBP4/Retinol Binding Protein 4, Polyclonal Antibody (Cat# AAA1750562)
Full Name
Anti-RBP4/Retinol Binding Protein 4 Picoband Antibody
Gene Names
Rbp4; Rbp-4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Western Blot (WB)
(Figure 1. Western blot analysis of ALDH1A3 using anti- ALDH1A3 antibody (MBS1750811).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates,Lane 2: mouse gaster tissue lysates,Lane 3: HEPG2 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ALDH1A3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALDH1A3 at approximately 56KD. The expected band size for ALDH1A3 is at 56KD. )
ALDH1A3, Polyclonal Antibody (Cat# AAA1750811)
Full Name
Anti-ALDH1A3 Picoband Antibody
Gene Names
ALDH1A3; ALDH6; MCOP8; RALDH3; ALDH1A6
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Edinburgh Minimal Media, Nitrogen Free, Culture Media (Cat# AAA652833)
Full Name
Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)
Purity
Molecular Biology Grade
Edinburgh Minimal Media Phosphate Free, Culture Media (Cat# AAA652754)
Full Name
Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)
Purity
Molecular Biology Grade
Western Blot (WB)
(Western blot detection of PRMT4/CARM1 in Hela, A431 and K562 cell lysates using PRMT4/CARM1 mouse mAb (1:200-1:500 diluted).Predicted band size:63KDa.Observed band size:63KDa.)
PRMT4/CARM1, Monoclonal Antibody (Cat# AAA475137)
Full Name
Anti-PRMT4/CARM1 Mouse mAb
Gene Names
CARM1; PRMT4
Reactivity
Human, Mouse
Applications
WB, IP
Purity
Affinity Purified
ELISA
(Indirect ELISA assay for anti-Ferritin Light Chain mouse mAb.Antigen coating concentration: 4ug/ml.)
Ferritin Light Chain, Monoclonal Antibody (Cat# AAA475160)
Full Name
Anti-Ferritin Light Chain Mouse mAb
Gene Names
FTL; LFTD; NBIA3
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Edinburgh Minimal Media, Culture Media (Cat# AAA653385)
Full Name
Edinburgh Minimal Media (EMM2) (Powder)
Purity
Molecular Biology Grade
Western Blot (WB)
(Western blot analysis of Cubilin expression in Human fetal kidney lysate (MBS179979).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUBN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CUBN )
Cubilin, Monoclonal Antibody (Cat# AAA179979)
Full Name
Anti-Cubilin Rabbit Monoclonal Antibody
Gene Names
CUBN; IFCR; MGA1; gp280
Reactivity
Human
Applications
IHC, WB
Purity
Affinity-chromatography
Testing Data
(Observed Ferritin Light Chain levels in Human serum samples at different dilution.)
Ferritin Light Chain, Monoclonal Antibody (Cat# AAA477198)
Full Name
Anti-Ferritin Light Chain Mouse mAb
Gene Names
FTL; LFTD; NBIA3
Reactivity
Human
Applications
EIA
Purity
Affinity Purified
Western Blot (WB)
(Western blot analysis of Cubilin expression in Human fetal kidney lysate (MBS179980).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUBN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CUBN )
Cubilin, Monoclonal Antibody (Cat# AAA179980)
Full Name
Anti-Cubilin Rabbit Monoclonal Antibody
Gene Names
CUBN; IFCR; MGA1; gp280
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Western Blot (WB)
(Figure 1. Western blot analysis of Ace using anti-Ace antibody (MBS1750358).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: mouse stomach tissue lysates,Lane 4: rat lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ace antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ace at approximately 180KD. The expected band size for Ace is at 150KD. )
Ace/Cd143, Polyclonal Antibody (Cat# AAA1750358)
Full Name
Anti-Ace/Cd143 Picoband antibody
Gene Names
Ace; CD143; AW208573
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Western Blot (WB)
(Western blot analysis of HMBS expression in HepG2 cell lysate (MBS179678).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HMBS )
HMBS, Monoclonal Antibody (Cat# AAA179678)
Full Name
Anti-HMBS Rabbit Monoclonal Antibody
Gene Names
HMBS; UPS; PBGD; PORC; PBG-D
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Western Blot (WB)
(Western blot analysis of AQP1 expression in human fetal kidney lysate (MBS179477).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AQP1 )
AQP1, Monoclonal Antibody (Cat# AAA179477)
Full Name
Anti-AQP1 Rabbit Monoclonal Antibody
Gene Names
AQP1; CO; CHIP28; AQP-CHIP
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Western Blot (WB)
(Western blot analysis of RPE65 expression in mouse eyeball lysate (MBS179464).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPE65 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPE65 )
RPE65, Monoclonal Antibody (Cat# AAA179464)
Full Name
Anti-RPE65 Rabbit Monoclonal Antibody
Gene Names
RPE65; p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Western Blot (WB)
(Western blot analysis of SERCA2 expression in HeLa cell lysate (MBS179578).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP2A2 )
SERCA2, Monoclonal Antibody (Cat# AAA179578)
Full Name
Anti-SERCA2 Rabbit Monoclonal Antibody
Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Western Blot (WB)
(Figure 1. Western blot analysis of HFE using anti-HFE antibody (MBS1750424).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HELA whole Cell lysates,Lane 2: HEPG2 whole Cell lysates,Lane 3: A431 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HFE antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HFE at approximately 40KD. The expected band size for HFE is at 40KD. )
HFE, Polyclonal Antibody (Cat# AAA1750424)
Full Name
Anti-HFE Picoband Antibody
Gene Names
HFE; HH; HFE1; HLA-H; MVCD7; TFQTL2
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Western Blot (WB)
(Western blot analysis of Aconitase 1 in (1) mouse kidney lysate; (2) HepG2 cell lysate (MBS179890).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACO1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACO1 )
Aconitase 1, Monoclonal Antibody (Cat# AAA179890)
Full Name
Anti-Aconitase 1 Rabbit Monoclonal Antibody
Gene Names
ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Western Blot (WB)
(Western blot analysis of Ferritin expression in Jurkat cell lysate (MBS179842).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTH1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FTH1 )
Ferritin, Monoclonal Antibody (Cat# AAA179842)
Full Name
Anti-Ferritin Rabbit Monoclonal Antibody
Gene Names
FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Immunohistochemistry (IHC)
FOLR2, Polyclonal Antibody (Cat# AAA5400404)
Full Name
FOLR2 Antibody
Reactivity
Mouse, Rat
Applications
EIA, IHC, IP, WB
Purity
Immobilized antigen affinity chromatography
