Edinburgh Minimal Media Phosphate Free Culture Media
Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)
Purity
Molecular Biology Grade
Synonyms
Edinburgh Minimal Media Phosphate Free; Edinburgh Minimal Media Phosphate Free (EMMP) (Powder); Edinburgh Minimal Media Phosphate Free culture media
Purity/Purification
Molecular Biology Grade
pH
6.5
Solubility
Colorless, clear, complete
Appearance
White, homogenous, free flowing powder.
Directions per Liter
Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.
Base
Ammonium Chloride: 5.000
Glucose: 20.000
Sodium Acetate•3H2O: 2.200
Glucose: 20.000
Sodium Acetate•3H2O: 2.200
Salt Stock
Calcium Chloride•2H2O: 0.0147
Magnesium Chloride•6H2O: 1.050
Potassium Chloride: 1.000
Sodium Sulfate: 0.040
Magnesium Chloride•6H2O: 1.050
Potassium Chloride: 1.000
Sodium Sulfate: 0.040
Vitamin Stock
Biotin: 0.00001
Inositol: 0.010
Nicotinic Acid: 0.010
Pantothenic Acid•Ca: 0.001
Inositol: 0.010
Nicotinic Acid: 0.010
Pantothenic Acid•Ca: 0.001
Mineral Stock
Boric Acid: 0.0005
Citric Acid: 0.001
Copper Sulfate•5H2O: 0.00004
Ferrous Chloride: 0.0002
Manganese Sulfate: 0.0004
Potassium Iodide: 0.0001
Molybdic Acid: 0.00004
Zinc Sulfate•7H2O: 0.0004
Citric Acid: 0.001
Copper Sulfate•5H2O: 0.00004
Ferrous Chloride: 0.0002
Manganese Sulfate: 0.0004
Potassium Iodide: 0.0001
Molybdic Acid: 0.00004
Zinc Sulfate•7H2O: 0.0004
Total
29.328g/Lit
Note
The above formulation was derived from the following referenced formula
Edinburgh Minimal Medium (EMM 2)
3g/L Potassium hydrogen phthalate (14.7mM)
2.2g/L Sodium phosphate dibasic (15.5mM)
5g/L Ammonium chloride (93.5mM)
2% (w/v) Glucose (111mM)
20ml/L Salts (50X stock)
1ml/L Vitamins (1000X stock)
0.1ml/L Minerals (10,000X stock)
2.2g/L Sodium phosphate dibasic (15.5mM)
5g/L Ammonium chloride (93.5mM)
2% (w/v) Glucose (111mM)
20ml/L Salts (50X stock)
1ml/L Vitamins (1000X stock)
0.1ml/L Minerals (10,000X stock)
50X Salts
52.5g/L Magnesium chloride•6H2O (0.26M)
0.735g/L Calcium chloride•2H2O (4.99mM)
50g/L Potassium chloride (0.67M)
2g/L Sodium sulfate (14.1mM)
0.735g/L Calcium chloride•2H2O (4.99mM)
50g/L Potassium chloride (0.67M)
2g/L Sodium sulfate (14.1mM)
1000X Vitamins
1g/L Pantothenic acid (4.20mM)
10g/L Nicotinic acid (81.2mM)
10g/L Inositol (55.5mM)
10mg/L Biotin (40.8uM)
10g/L Nicotinic acid (81.2mM)
10g/L Inositol (55.5mM)
10mg/L Biotin (40.8uM)
10,000X Minerals
5g/L Boric acid (SO.9mM)
4g/L Manganese sulfate (23.7mM) )
4g/L Zinc sulfate•7H20 (13.9mM) )
2g/L Ferrous Chloride (7AOmM) )
0.4g/L Molybdic acid (2A7mM) )
1 g/L Potassium iodide (6.02mM) )
0.4g/L Cupric sulfate.5H20 (1.60mM) )
10g/L Citric acid (47.6mM)
4g/L Manganese sulfate (23.7mM) )
4g/L Zinc sulfate•7H20 (13.9mM) )
2g/L Ferrous Chloride (7AOmM) )
0.4g/L Molybdic acid (2A7mM) )
1 g/L Potassium iodide (6.02mM) )
0.4g/L Cupric sulfate.5H20 (1.60mM) )
10g/L Citric acid (47.6mM)
Preparation and Storage
Store powdered media ar RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Edinburgh Minimal Media Phosphate Free culture media
Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.
Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.
Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.
Product Categories/Family for Edinburgh Minimal Media Phosphate Free culture media
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Product Notes
The Edinburgh Minimal Media Phosphate Free (Catalog #AAA652754) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Edinburgh Minimal Media Phosphate Free, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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