Edinburgh Minimal Media, Nitrogen Free Culture Media

Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)

Cat. Num. AAA652833

• Purity: Molecular Biology Grade
• Synonyms: Edinburgh Minimal Media; Nitrogen Free; Nitrogen Free (EMM2) (Powder); Nitrogen Free culture media

• Purity/Purification: Molecular Biology Grade
• Form/Format: White, homogeneous, free flowing powder

• Solubility: Colorless, clear, complete
• pH: 5.9
• Directions per Liter: Dissolve 27.33g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
• Note: The above formulation was derived from the following referenced formula:; Edinburgh Minimal Medium (EMM 2) -- ; 3g/L: Potassium hydrogen phthalate (14.7mM); 2.2g/L: Sodium phosphate dibasic (15.5mM); 5g/L: Ammonium chloride (93.5mM); 2% (w/v): Glucose(111mM); 20ml/L: Salts (50X stock); 1ml/L: Vitamins (1000X stock); 0.1ml/L: Minerals (10,000X stock); 50X Salts --; 52.5g/L: Magnesium chloride•6H2O (0.26M); 0.735g/L: Calcium chloride•2H2O (4.99mM); 50g/L: Potassium chloride (0.67M); 2g/L: Sodium sulfate (14.1mM); 1000X Vitamins --; 1g/L: Pantothenic acid (4.20mM); 10g/L: Nicotinic acid (81.2mM); 10g/L: Inositol (55.5mM); 10mg/L Biotin (40.8uM); 10,000X Minerals --; 5g/L: Boric acid (80.9mM); 4g/L: Manganese sulfate (23.7mM); 4g/L: Zincsulfate•7H2O (13.9mM); 2g/L: Ferric chloride•6H2O (7.40mM); 0.4g/L: Molybdic acid (2.47mM); 1g/L: Potassium iodide (6.02mM); 0.4g/L: Cupric sulfate.5H2O (1.60mM); 10g/L: Citric acid (47.6mM)
• Components shown as g/Liter: Base --; Potassium Hydrogen Phthalate: 3.00; Sodium Phosphate Dibasic: 2.20; Ammonium Chloride: Absent; Glucose: 20.00; Salt Stock --; Magnesium Chloride•6H2O: 1.05; Calcium Chloride•2H2O: 0.0147; Potassium Chloride: 1.00; Sodium Sulfate: 0.04; Vitamin Stock --; Pantothenic Acid: 0.001; Nicotinic Acid: 0.01; Inositol: 0.01; Biotin: 0.00001; Mineral Stock: --; Boric Acid: 0.0005; Manganese Sulfate: 0.0004; Zinc Sulfate•7H2O: 0.0004; Ferrous Chloride: 0.0002; Molybdic Acid: 0.00004; Potassium Iodide: 0.0001; Copper Sulfate •5H2O: 0.00004; Citric Acid: 0.001; Total: 27.33g/Liter
• Preparation and Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Related Product Information for Edinburgh Minimal Media, Nitrogen Free culture media

Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. EMM2 is used for vegetative growth. This formulation does not contain Ammonium Chloride, a Nitrogen source.

Product Categories/Family for Edinburgh Minimal Media, Nitrogen Free culture media

Culture Media; Media-Yeast