Western Blot (WB)
(Western Blot analysis of Various lysates using EGFR Monoclonal Antibody at dilution of 1:20000.)
Mouse anti-Human EGFR Monoclonal Antibody | anti-EGFR antibody
EGFR Monoclonal Antibody
IHC: 1:400-1:1600
Western Blot (WB)
(Western Blot analysis of Various lysates using EGFR Monoclonal Antibody at dilution of 1:20000.)
Western Blot (WB)
(Western Blot analysis of Various lysates using EGFR Monoclonal Antibody at dilution of 1:20000.)
NCBI and Uniprot Product Information
Observed Molecular Weight: 145-165kDa
NCBI Description
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Uniprot Description
EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.
Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tumor suppressor
Chromosomal Location of Human Ortholog: 7p11.2
Cellular Component: AP-2 adaptor complex; apical plasma membrane; basolateral plasma membrane; cell surface; clathrin-coated vesicle membrane; cytoplasm; early endosome membrane; endoplasmic reticulum membrane; endosome; endosome membrane; extracellular space; focal adhesion; Golgi membrane; integral component of membrane; membrane; membrane raft; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; receptor complex; Shc-EGFR complex; synapse
Molecular Function: actin filament binding; ATP binding; cadherin binding; calmodulin binding; chromatin binding; double-stranded DNA binding; enzyme binding; epidermal growth factor binding; epidermal growth factor receptor activity; identical protein binding; integrin binding; MAP kinase kinase kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; protein phosphatase binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; signal transducer, downstream of receptor, with protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; ubiquitin protein ligase binding; viral receptor activity
Biological Process: activation of MAPKK activity; activation of phospholipase C activity; astrocyte activation; calcium-dependent phospholipase A2 activation; cell proliferation; cell surface receptor signaling pathway; cellular response to cadmium ion; cellular response to dexamethasone stimulus; cellular response to epidermal growth factor stimulus; cellular response to estradiol stimulus; cellular response to reactive oxygen species; cerebral cortex cell migration; circadian rhythm; digestive tract morphogenesis; diterpenoid metabolic process; embryonic placenta development; entry of virus into host cell; epidermal growth factor receptor signaling pathway; ERBB2 signaling pathway; hair follicle development; hydrogen peroxide metabolic process; learning and/or memory; lung development; magnesium ion homeostasis; MAPK cascade; midgut development; negative regulation of apoptosis; negative regulation of cardiocyte differentiation; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of ERBB signaling pathway; negative regulation of mitotic cell cycle; negative regulation of protein catabolic process; neuron projection morphogenesis; ossification; ovulation cycle; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; phosphatidylinositol phosphorylation; positive regulation of blood vessel diameter; positive regulation of bone resorption; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle; positive regulation of DNA repair; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of MAP kinase activity; positive regulation of NIK/NF-kappaB signaling; positive regulation of nitric oxide biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphorylation; positive regulation of prolactin secretion; positive regulation of protein kinase B signaling; positive regulation of protein kinase C activity; positive regulation of protein phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of superoxide anion generation; positive regulation of synaptic transmission, glutamatergic; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of vasoconstriction; protein autophosphorylation; protein insertion into membrane; regulation of cell motility; regulation of ERK1 and ERK2 cascade; regulation of JNK cascade; regulation of nitric-oxide synthase activity; regulation of peptidyl-tyrosine phosphorylation; regulation of phosphatidylinositol 3-kinase signaling; regulation of transcription from RNA polymerase II promoter; response to calcium ion; response to cobalamin; response to hydroxyisoflavone; response to osmotic stress; response to stress; salivary gland morphogenesis; signal transduction; tongue development; translation; wound healing
Disease: Inflammatory Skin And Bowel Disease, Neonatal, 2; Lung Cancer
Research Articles on EGFR
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Product Notes
The EGFR egfr (Catalog #AAA2549069) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EGFR Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:10000-1:20000 IHC: 1:400-1:1600. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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