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272 results for " Rat tPA" - showing 1-50
Testing Data
Tissue Plasminogen Activator (tPA), Antibody (Cat# AAA315411)
Full Name
Rabbit anti Mouse tPA
Gene Names
PLAT; TPA; T-PA; DKFZp686I03148
Applications
EIA/ELISA, Western Blot
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Purity
Protein A chromatography
Pricing
$480/0.2 mg | $2,015/5x0.2 mg
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)
Erk1/2-T202/Y204, Antibody (Cat# AAA9127838)
Full Name
Phospho-Erk1/2-T202/Y204 Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Plasminogen Activator, Tissue, ELISA Kit (Cat# AAA90201)
Full Name
Rat Plasminogen Activator, Tissue ELISA Kit
Reactivity
Rat
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western blot detection of c-Fos in HCT116, COS7 and C6 cell lysates using c-Fos mouse mAb (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.)
c-Fos, Monoclonal Antibody (Cat# AAA475305)
Full Name
Anti-c-Fos Mouse mAb
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human, Rat, Monkey
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MEK1-S217/MEK2-S221 antibody. NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
MAP2K1/MAP2K2-S217/S221, Antibody (Cat# AAA128486)
Full Name
Phospho-MAP2K1/MAP2K2-S217/S221 Polyclonal Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$210/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot detection of c-Fos antibody in HCT116,COS7 and C6 cell lysates using c-Fos antibody (1ïš500 diluted).Predicted band size:62KDa.Observed band size:62KDa.)
c-Fos, Monoclonal Antibody (Cat# AAA9400504)
Full Name
c-Fos Monoclonal Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human, Rat, Monkey
Applications
WB
Purity
Affinity purified
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of 293 cells, using Phospho-MAP2K1-T286 antibody at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
MAP2K1-pT285, Antibody (Cat# AAA125269)
Full Name
Phospho-MAP2K1-pT285
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
tPA, ELISA Kit (Cat# AAA2504852)
Full Name
Rat tPA (Plasminogen Activator, Tissue) ELISA Kit
Gene Names
PLAT; TPA; T-PA
Reactivity
Rat
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
TIMP-4, ELISA Kit (Cat# AAA2508495)
Full Name
Rat TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA Kit
Reactivity
Rat
Pricing
$335/48-Strip-Wells | $385/96-Strip-Wells | $1,605/5x96-Strip-Wells | $3,155/10x96-Strip-Wells | $215/24-Strip-Wells
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (βII Ser660) antibody at 1:1000 dilution. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
Phospho-PKCalpha/beta II-S657, Antibody (Cat# AAA9129000)
Full Name
Phospho-PKCalpha/beta II-S657 Polyclonal Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ATP1 alpha1/Na+K+ ATPase1 phosphorylation expression in Rat brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)
ATP1 alpha1/Na+K+ ATPase1, Polyclonal Antibody (Cat# AAA9600881)
Full Name
Phospho-ATP1 alpha1/Na+K+ ATPase1 (Ser23) Antibody
Gene Names
Atp1a1; Nkaa1b
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Plasminogen, Polyclonal Antibody (Cat# AAA2026610)
Full Name
Polyclonal Antibody to Plasminogen Activator, Tissue (tPA)
Gene Names
Plat; tPA; PATISS
Reactivity
Rat
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $340/0.5 mL | $415/1 mL | $1,205/5x1 mL
Western Blot (WB)
(Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
ERK3-S189, Antibody (Cat# AAA125434)
Full Name
Phospho-ERK3-S189 Polyclonal Antibody
Gene Names
MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Immunohistochemistry (IHC)
(MBS9600653 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Bim, Polyclonal Antibody (Cat# AAA9600653)
Full Name
Phospho-Bim (Ser77) Antibody
Gene Names
BCL2L11; BAM; BIM; BOD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKD/PKCmu-S744/748 pAb(MBS9129009) at 1:1000 dilution or PRKD1 antibody (A16185).NIH/3T3 cells were treated by PMA/TPA(200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Phospho-PKD/PKCmu-S744/748, Antibody (Cat# AAA9129009)
Full Name
Phospho-PKD/PKCmu-S744/748 Polyclonal Antibody
Gene Names
PRKD1; PKD; PKCM; PRKCM; PKC-MU
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600652 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Bim, Polyclonal Antibody (Cat# AAA9600652)
Full Name
Phospho-Bim (Ser69) Antibody
Gene Names
BCL2L11; BAM; BIM; BOD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RAF1-pS338 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)
Phospho-RAF1-pS338, Antibody (Cat# AAA9129018)
Full Name
Phospho-RAF1-pS338 Polyclonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of Raji cells, using Phospho-BRAF-S445 antibody at 1:2000 dilution. Raji cells were treated by PMA/TPA (200nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
BRAF-S445, Antibody (Cat# AAA125740)
Full Name
Phospho-BRAF-S445
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Host: RabbitTarget Name: PLATSample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)
PLAT, Polyclonal Antibody (Cat# AAA3213503)
Full Name
PLAT antibody - middle region
Gene Names
PLAT; TPA; T-PA
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Activity Data
(Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Plasminogen (Plg), Active Protein (Cat# AAA2097291)
Full Name
Active Plasminogen (Plg)
Gene Names
Plg; Ab1-346
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$215/0.01 mg | $450/0.05 mg | $690/0.1 mg | $850/0.2 mg | $1,660/0.5 mg | $2,470/1 mg | $6,035/5 mg
Western Blot (WB)
(Western blot analysis of extracts of HEL cells treated with TPA, using Phospho-AML1 (Ser249) Antibody.)
AML1, Polyclonal Antibody (Cat# AAA9600644)
Full Name
Phospho-AML1 (Ser249) Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600634 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CX43, Polyclonal Antibody (Cat# AAA9600634)
Full Name
Phospho-CX43(Tyr265) Antibody
Gene Names
GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-STMN1-S25 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
STMN1-S25, Antibody (Cat# AAA128550)
Full Name
Phospho-STMN1-S25 Polyclonal Antibody
Gene Names
STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from rat liver, using PLAT Antibody.)
PLAT, Polyclonal Antibody (Cat# AAA9608078)
Full Name
PLAT Antibody
Gene Names
PLAT; TPA; T-PA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600694 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MSK1, Polyclonal Antibody (Cat# AAA9600694)
Full Name
Phospho-MSK1 (Ser360) Antibody
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IFRD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IFRD1, Polyclonal Antibody (Cat# AAA9126897)
Full Name
IFRD1
Gene Names
IFRD1; PC4; TIS7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of ITGAX expression in Ant TPA lysate ;, The lane on the left is treated with the antigen-specific peptide.)
ITGAX, Polyclonal Antibody (Cat# AAA9604056)
Full Name
ITGAX Antibody
Gene Names
ITGAX; CD11C; SLEB6
Reactivity
Human, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of MED15 using NIH-3T3 whole cell lysates)
MED15, Polyclonal Antibody (Cat# AAA9609930)
Full Name
MED15 Antibody
Gene Names
MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-GPRC5A / RAI3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 4-10 ug/ml.)
GPRC5A / RAI3, Polyclonal Antibody (Cat# AAA247489)
Full Name
Rabbit Polyclonal to Human GPRC5A / RAI3
Gene Names
GPRC5A; RAI3; TIG1; RAIG1; GPCR5A; PEIG-1
Reactivity
Gorilla, Human
Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin, ICC
Purity
Immunoaffinity Purified
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Immunohistochemistry (IHC)
(MBS9600650 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
BCL9L, Polyclonal Antibody (Cat# AAA9600650)
Full Name
Phospho-BCL9L (Ser915) Antibody
Gene Names
BCL9L; BCL9-2; DLNB11
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600727 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
RSK2, Polyclonal Antibody (Cat# AAA9600727)
Full Name
Phospho-RSK2 (Ser227) Antibody
Gene Names
RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa and C6 cells, using Phospho-PEA15-S104 antibody at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 10s.)
PEA15-S104, Polyclonal Antibody (Cat# AAA9135641)
Full Name
Phospho-PEA15-S104 pAb
Gene Names
PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa and 293T cells, using Phospho-RPS6KB1-S235/236 antibody at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Phospho-RPS6-S235/236, Antibody (Cat# AAA9129011)
Full Name
Phospho-RPS6-S235/236 Polyclonal Antibody
Gene Names
RPS6; S6
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-GPRC5A / RAI3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 18-36 ug/ml.)
GPRC5A / RAI3, Polyclonal Antibody (Cat# AAA242985)
Full Name
Rabbit Polyclonal to Human GPRC5A / RAI3
Gene Names
GPRC5A; RAI3; TIG1; RAIG1; GPCR5A; PEIG-1
Reactivity
Gibbon, Human
Predicted Reactivity: Monkey, Mouse (at least 90% immunogen sequence identity)
Predicted Reactivity: Monkey, Mouse (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin, ICC
Purity
Immunoaffinity Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-VIM-S39 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
VIM-S39, Polyclonal Antibody (Cat# AAA9135659)
Full Name
Phospho-VIM-S39 pAb
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of IFRD1 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IFRD1, Polyclonal Antibody (Cat# AAA9603907)
Full Name
IFRD1 Antibody
Gene Names
IFRD1; PC4; TIS7
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa serum-starved treated with TPA, using Phospho-FOS (Ser32) Antibody.)
FOS, Polyclonal Antibody (Cat# AAA9600630)
Full Name
Phospho-FOS (Ser32) Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-MAPK3-Y204 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
MAPK3-Y204, Antibody (Cat# AAA128336)
Full Name
Phospho-MAPK3-Y204 Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of MED15 using anti-MED15 antibody (MBS1750843).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates,Lane 2: human U2OS whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human 22RV1 whole cell lysates,Lane 5: human K562 whole cell lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED15 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED15 at approximately 87KD. The expected band size for MED15 is at 87KD. )
MED15/Pcqap, Polyclonal Antibody (Cat# AAA1750843)
Full Name
Anti-MED15/Pcqap Picoband Antibody
Gene Names
MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-MAPK3-T202 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 30s.)
MAPK3-T202, Antibody (Cat# AAA128246)
Full Name
Phospho-MAPK3-T202 Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-MAP2K1-pS221 antibody at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.)
MAP2K1-pS221, Antibody (Cat# AAA125442)
Full Name
Phospho-MAP2K1-pS221 Polyclonal Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-SRF-S103 antibody at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)
SRF-S103, Polyclonal Antibody (Cat# AAA9135682)
Full Name
Phospho-SRF-S103 pAb
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of C6 cells, using Phospho-GRIN1-S890 antibody at 1:1000 dilution. C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
GRIN1-S890, Polyclonal Antibody (Cat# AAA9135679)
Full Name
Phospho-GRIN1-S890 pAb
Gene Names
GRIN1; NR1; MRD8; GluN1; NMDA1; NDHMSD; NDHMSR; NMD-R1; NMDAR1
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-PLCG1-S1248 antibody at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
PLCG1-S1248, Polyclonal Antibody (Cat# AAA9135680)
Full Name
Phospho-PLCG1-S1248 pAb
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of A-431 and HeLa cells, using Phospho-MAP3K5-S1033 antibody at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
MAP3K5-S1033, Antibody (Cat# AAA125910)
Full Name
Phospho-MAP3K5-S1033
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of 231 cell using PLAT Polyclonal Antibody at dilution of 1:450)
PLAT, Polyclonal Antibody (Cat# AAA2516449)
Full Name
PLAT Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of 293 and NIH/3T3 cells, using Phospho-MAP2K1-S298 antibody at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
MAP2K1-S297, Antibody (Cat# AAA125547)
Full Name
Phospho-MAP2K1-S297 Polyclonal Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells treated with UV/TPA, using Phospho-MAPKAPK-2 (Thr222) Antibody.)
MAPKAPK-2, Polyclonal Antibody (Cat# AAA9600689)
Full Name
Phospho-MAPKAPK-2 (Thr222) Antibody
Gene Names
MAPKAPK2; MK2; MK-2; MAPKAP-K2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of C6 cells, using Phospho-RPS6KA1-T359 antibody at 1:1000 dilution. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 degree C for 1 hour.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30 degree C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.)
RPS6KA1-T359, Polyclonal Antibody (Cat# AAA9135620)
Full Name
Phospho-RPS6KA1-T359 pAb
Gene Names
RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from TNF-alpha and TPA treated A549 cell lysates, using GCP2 Antibody. The lane on the left was treated with blocking peptide.)
GCP2, Polyclonal Antibody (Cat# AAA9612229)
Full Name
GCP2 Antibody
Gene Names
CXCL6; GCP2; CKA-3; GCP-2; SCYB6
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(100%)
Predicted Reactivity: Bovine(100%)
Applications
EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
