Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKD/PKCmu-S744/748 pAb(MBS9129009) at 1:1000 dilution or PRKD1 antibody (A16185).NIH/3T3 cells were treated by PMA/TPA(200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Rabbit Phospho-PKD/PKCmu-S744/748 Antibody | anti-S744/748 antibody
Phospho-PKD/PKCmu-S744/748 Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKD/PKCmu-S744/748 pAb(MBS9129009) at 1:1000 dilution or PRKD1 antibody (A16185).NIH/3T3 cells were treated by PMA/TPA(200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKD/PKCmu-S744/748 pAb(MBS9129009) at 1:1000 dilution or PRKD1 antibody (A16185).NIH/3T3 cells were treated by PMA/TPA(200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
NCBI and Uniprot Product Information
Observed MW: 102 kDa
NCBI Description
PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010]
Uniprot Description
PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A
Protein type: Autophagy; Protein kinase, CAMK; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Kinase, protein; CAMK group; PKD family
Chromosomal Location of Human Ortholog: 14q11
Cellular Component: cell cortex; cytoplasm; cytosol; Golgi apparatus; integral to plasma membrane; intercellular junction; nucleus; plasma membrane; trans-Golgi network
Molecular Function: ATP binding; identical protein binding; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity
Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; angiogenesis; apoptosis; cell proliferation; Golgi organization and biogenesis; Golgi vesicle transport; inflammatory response; innate immune response; integrin-mediated signaling pathway; negative regulation of endocytosis; peptidyl-serine phosphorylation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; regulation of release of sequestered calcium ion into cytosol; signal transduction; sphingolipid biosynthetic process; sphingolipid metabolic process; vascular endothelial growth factor receptor signaling pathway
Research Articles on S744/748
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Product Notes
The S744/748 prkd1 (Catalog #AAA9129009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospho-PKD/PKCmu-S744/748 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the S744/748 prkd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PKD/PKCmu-S744/748, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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