Rabbit ERK3-S189 Antibody | anti-MAPK6 antibody

Phospho-ERK3-S189 Polyclonal Antibody

Cat. Num. AAA125434

• Gene Names: MAPK6; ERK3; PRKM6; p97MAPK; HsT17250
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: ERK3-S189; Phospho-ERK3-S189 Polyclonal Antibody; Phospho-MAPK6-S189: DKFZp686F03189; ERK3; HsT17250; PRKM6; p97MAPK; MAPK6; anti-MAPK6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 721

• Applicable Applications for anti-MAPK6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Species: Human
• Route: Synthetic Peptide
• Immunogen: A phospho specific peptide corresponding to residues surrounding S189 of human ERK3
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

Related Product Information for anti-MAPK6 antibody

Erk3, also known as MAPK6 or p97 MAPK, is almost 50% identical to Erk1/2 at the kinase domain located in its amino-terminal region (1). However, Erk3 is distinguished from other MAP kinases in that it lacks the conserved TXY motif in its activation loop, possessing instead an SEG motif (1,2). Phosphorylation at Ser189 in the SEG motif has been reported (2,3). With limited information about its upstream kinases and downstream substrates, the significance of this phosphorylation remains to be elucidated (3,4). Erk3 is an inherently unstable protein, rapidly degraded through amino-terminal ubiquitination and proteasome degradation (3,5). A site-specific cleavage, depending on a short stretch of acidic residues of Erk3, might regulate its translocation from the Golgi/ERGIC to the nucleus during the cell cycle (6). Accumulating evidence suggests that Erk3 is involved in cell differentiation (1,3,6).

Product Categories/Family for anti-MAPK6 antibody

Phosphorylation

NCBI and Uniprot Product Information

• NCBI GI #: 2499596
• NCBI GeneID: 5597
• UniProt Accession #: Q16659; Q68DH4; Q8IYN8; B2R945; B5BU65
• Molecular Weight: 721
• NCBI Official Full Name: Mitogen-activated protein kinase 6
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 6
• NCBI Official Symbol: MAPK6
• NCBI Official Synonym Symbols: ERK3; PRKM6; p97MAPK; HsT17250
• NCBI Protein Information: mitogen-activated protein kinase 6; ERK-3; MAPK 6; p97-MAPK; MAP kinase 6; MAP kinase isoform p97; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97
• UniProt Protein Name: Mitogen-activated protein kinase 6
• UniProt Gene Name: MAPK6
• UniProt Synonym Gene Names: ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
• UniProt Entry Name: MK06_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; ATP binding

Biological Process: MAPKKK cascade; cell cycle; signal transduction; protein amino acid phosphorylation