Mouse Uteroglobin ELISA Kit | Scgb1a1 elisa kit

Mouse Uteroglobin ELISA Kit

Cat. Num. AAA761923

• Gene Names: SCGB1A1; UGB; UP1; CC10; CC16; CCSP; UP-1; CCPBP
• Reactivity: Mouse
• Synonyms: Uteroglobin; Mouse Uteroglobin ELISA Kit; Scgb1a1/CC16/Blastokinin/CC10/CCPBP/CCSP/Clara/PCB-BP/SCGB1A1/UGB/blastokinin/CC10Urine protein 1/CC16/CCSPCCPBP/Clara cell phospholipid-binding protein/Clara cells 10 kDa secretory protein/Secretoglobin family 1A member 1/secretoglobin; family 1A; member 1 (uteroglobin)/UGBUP-1/UP1/Urinary protein 1/uteroglobin; Scgb1a1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of sCD80. No significant cross-reactivity or interference between sCD80 and analogues was observed.
• Sequence Length: 91

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: <46.875pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level sCD80 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level sCD80 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Scgb1a1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-sCD80 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-sCD80 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the sCD80 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of sCD80 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507809
• NCBI GeneID: 7356
• NCBI Accession #: NP_003348.1
• NCBI GenBank Nucleotide #: NM_003357.4
• Molecular Weight: 9,994 Da
• NCBI Official Full Name: uteroglobin
• NCBI Official Synonym Full Names: secretoglobin family 1A member 1
• NCBI Official Symbol: SCGB1A1
• NCBI Official Synonym Symbols: UGB; UP1; CC10; CC16; CCSP; UP-1; CCPBP
• NCBI Protein Information: uteroglobin
• UniProt Protein Name: Uteroglobin
• Protein Family: Uteroglobin
• UniProt Gene Name: SCGB1A1
• UniProt Synonym Gene Names: CC10; CCSP; UGB; CCPBP; CC10; UP-1; UP1; Urine protein 1
• UniProt Entry Name: UTER_HUMAN

NCBI Description

This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma. [provided by RefSeq, May 2010]

Uniprot Description

SCGB1A1: Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2. Belongs to the secretoglobin family.

Protein type: Secreted, signal peptide; Inhibitor; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: extracellular space; nuclear envelope; rough endoplasmic reticulum; secretory granule

Molecular Function: phospholipase A2 inhibitor activity; protein binding

Biological Process: embryo implantation; female pregnancy; negative regulation of catalytic activity; negative regulation of interferon-gamma production; negative regulation of interleukin-13 production; negative regulation of interleukin-4 production; negative regulation of interleukin-5 production; negative regulation of T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; regulation of inflammatory response; regulation of mRNA stability; response to cytokine stimulus; response to drug; response to glucocorticoid stimulus; response to lipopolysaccharide; response to ozone; response to silicon dioxide; response to xenobiotic stimulus; signal transduction

Disease: Asthma, Susceptibility To