Chicken ALDOC ELISA Kit | ALDOC elisa kit

Chicken ALDOC (Aldolase C, Fructose Bisphosphate) ELISA Kit

Cat. Num. AAA2505894

• Gene Names: ALDOC; ALDC
• Reactivity: Chicken
• Synonyms: ALDOC; Chicken ALDOC (Aldolase C; Fructose Bisphosphate) ELISA Kit; ALDOC elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken ALDOC. No significant cross-reactivity or interference between Chicken ALDOC and analogues was observed.
• Sequence Length: 364

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALDOC elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken ALDOC concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ALDOC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ALDOC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ALDOC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ALDOC. You can calculate the concentration of ALDOC in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49456717
• NCBI GeneID: 230
• UniProt Accession #: P09972; Q3SYL3; Q6FH94; Q6P0L5; B2R5R3
• Molecular Weight: 39,456 Da
• NCBI Official Full Name: ALDOC, partial
• NCBI Official Synonym Full Names: aldolase C, fructose-bisphosphate
• NCBI Official Symbol: ALDOC
• NCBI Official Synonym Symbols: ALDC
• NCBI Protein Information: fructose-bisphosphate aldolase C; aldolase 3; brain-type aldolase; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase
• UniProt Protein Name: Fructose-bisphosphate aldolase C
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: ALDOC
• UniProt Synonym Gene Names: ALDC
• UniProt Entry Name: ALDOC_HUMAN

NCBI Description

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Uniprot Description

ALDOC: a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - fructose and mannose; Lyase; EC 4.1.2.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 17cen-q12

Cellular Component: cytoskeleton; mitochondrion; axon; cytosol

Molecular Function: protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity

Biological Process: fructose 1,6-bisphosphate metabolic process; organ regeneration; glycolysis; epithelial cell differentiation; apoptosis; carbohydrate metabolic process; response to hypoxia; glucose metabolic process; protein heterotetramerization; pathogenesis; response to organic nitrogen; response to organic cyclic substance; protein homotetramerization; gluconeogenesis; fructose metabolic process; aging