SAP18 recombinant protein

SAP18, 20- 172aa, Human, His tag, E Coli

Cat. Num. AAA203341

• Gene Names: SAP18; SAP18P; 2HOR0202
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SAP18; 20- 172aa; Human; His tag; E Coli; Sin3A-associated protein 18kDa; Sin3A-associated protein; 2HOR0202; SAP18P; 18kDa Histone deacetylase complex subunit SAP18; Sin3-associated polypeptide p18.; SAP18 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY
• Sequence Length: 172

• Applicable Applications for SAP18 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SAP18 recombinant protein

SAP18, also known as Sin3A-associated protein, is component of the histone deacetylase complex that plays an important role in the regulation of eukaryotic gene expression. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. It also has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. Recombinant SAP18 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SAP18 recombinant protein

Transcription & Translation

References

Zhang Y., et al. (1997) Cell. 89 (3):357-64. ; Matyash A, et al. (2009) J Biol Chem. 284 (5):3012-20.;

NCBI and Uniprot Product Information

• NCBI GI #: 215490089
• NCBI GeneID: 10284
• NCBI Accession #: NP_005861
• NCBI GenBank Nucleotide #: NM_005870.4
• UniProt Accession #: O00422; Q2TTR4; Q6IAW9; Q8N606; Q9UF14; B2R494
• Molecular Weight: 19.7 kDa (173 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: histone deacetylase complex subunit SAP18
• NCBI Official Synonym Full Names: Sin3A associated protein 18
• NCBI Official Symbol: SAP18
• NCBI Official Synonym Symbols: SAP18P; 2HOR0202
• NCBI Protein Information: histone deacetylase complex subunit SAP18
• UniProt Protein Name: Histone deacetylase complex subunit SAP18
• Protein Family: Zinc finger A20 domain-containing stress-associated protein
• UniProt Gene Name: SAP18

NCBI Description

Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008]

Uniprot Description

Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function.