Glutamate Decarboxylase 2, Acid (GAD2) Recombinant Protein | GAD2 recombinant protein

Recombinant Glutamate Decarboxylase 2, Acid (GAD2)

Cat. Num. AAA2010504

• Gene Names: Gad2; gad65
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Glutamate Decarboxylase 2; Acid (GAD2); Recombinant Glutamate Decarboxylase 2; GAD2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MGHHHHHHSGSEF-MVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MLIARYKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EVKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMS
• Sequence Length: 585

• Applicable Applications for GAD2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met188~Ser374 (Accession # Q05683) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978871
• NCBI GeneID: 24380
• NCBI Accession #: NP_036695.1
• NCBI GenBank Nucleotide #: NM_012563.1
• UniProt Accession #: Q05683
• Molecular Weight: 21.8kDa
• NCBI Official Full Name: glutamate decarboxylase 2
• NCBI Official Synonym Full Names: glutamate decarboxylase 2
• NCBI Official Symbol: Gad2
• NCBI Official Synonym Symbols: gad65
• NCBI Protein Information: glutamate decarboxylase 2; GAD-65; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; Glutamate decarboxylase 2 (islet); 65 kDa glutamic acid decarboxylase; glutamate decarboxylase 65 kDa isoform
• UniProt Protein Name: Glutamate decarboxylase 2
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: Gad2
• UniProt Synonym Gene Names: Gad65; GAD-65
• UniProt Entry Name: DCE2_RAT

NCBI Description

catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006]

Uniprot Description

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate; Lyase; Other Amino Acids Metabolism - beta-alanine

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; cytoplasm; synapse; cytoplasmic vesicle; cell junction; cytosol

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process