Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SAE1/UBA2 Complex Recombinant Protein | SAE1/UBA2 recombinant protein
SAE1/UBA2 Complex, Active
Applications
Western Blot
Synonyms
SAE1/UBA2 Complex; Active; SAE1: UBLE1A; SUA1; HSPC140; AOS1; FLJ3091 UBA2: SAE2; UBLE1B; SAE2; ARX; HRIHFB2115; SAE1/UBA2 recombinant protein
Host
Sf9 insect cells.
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for SAE1/UBA2 recombinant protein
Ubiquitin Enzyme Assay, Western Blot (WB)
Tag
His/GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for SAE1/UBA2 recombinant protein
Recombinant full-length human SAE1 (UBLE1A) and human UBA2 (SAE2) were co-expressed by baculovirus in Sf9 insect cells. SAE1 used an N-terminal his tag, and the gene accession number is NM_005500. UBA2 used an N-terminal GST tag, and the gene accession number is NM_005499.
SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins to regulate protein structures and intracellular localization (1). The SAE1/SAE2 dimer functions in SUMO1 activation in a manner analogous to the single E1 ubiquitin-activating enzymes (2). The SAE2 inactivation may be a therapeutic strategy in MYC-driven cancers (3).
SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins to regulate protein structures and intracellular localization (1). The SAE1/SAE2 dimer functions in SUMO1 activation in a manner analogous to the single E1 ubiquitin-activating enzymes (2). The SAE2 inactivation may be a therapeutic strategy in MYC-driven cancers (3).
Product Categories/Family for SAE1/UBA2 recombinant protein
References
1. Okuma, T. et.al: In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem. Biophys. Res. Commun. 254: 693-698, 1999.
2. Desterro, J. et. al: Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem. 274: 10618-10624, 1999.
3. Kessler, J. D. et.al: A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 335: 348-353, 2012.
2. Desterro, J. et. al: Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem. 274: 10618-10624, 1999.
3. Kessler, J. D. et.al: A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 335: 348-353, 2012.
NCBI and Uniprot Product Information
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Product Notes
The SAE1/UBA2 (Catalog #AAA517742) is a Recombinant Protein produced from Sf9 insect cells. and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SAE1/UBA2 Complex can be used in a range of immunoassay formats including, but not limited to, Ubiquitin Enzyme Assay, Western Blot (WB). Researchers should empirically determine the suitability of the SAE1/UBA2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAE1/UBA2 Complex, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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