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PLK4 (G95L) Recombinant Protein | PLK4 recombinant protein
PLK4 (G95L), Active
Synonyms
PLK4 (G95L); Active; SAK; STK18; PLK4 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT and 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Activity
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SDS-Page
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SDS-Page
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Related Product Information for PLK4 recombinant protein
Recombinant human PLK4 (G95L)(1-836) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PLK4 gene accession number is NM_014264.
PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggers the simultaneous formation of multiple procentrioles around each preexisting centriole resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells (1). The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development (2).
PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggers the simultaneous formation of multiple procentrioles around each preexisting centriole resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells (1). The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development (2).
Product Categories/Family for PLK4 recombinant protein
References
1. Kleylein-Sohn, J. et.al: Plk4-induced centriole biogenesis in human cells. Dev. Cell 13: 190-202, 2007. 2. Ko, M. A. et.al: Plk4 haploinsufficiency causes mitotic infidelity and carcinogenesis. Nature Genet. 37: 883-888, 2005.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
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Product Notes
The PLK4 (Catalog #AAA517424) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PLK4 (G95L), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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