Typical Testing Data/Standard Curve (for reference only)

Mouse MRP14 ELISA Kit | S100A9 elisa kit

Mouse MRP14 ELISA Kit

Gene Names

S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964

Reactivity

Mouse

Applications

ELISA

Synonyms

MRP14; Mouse MRP14 ELISA Kit; CAGB; CFAG; Protein S100-A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9; S100A9 elisa kit

Ordering
Cat. Num. AAA8244633 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Reactivity

Mouse

Specificity

The Mouse MRP14 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse MRP14 proteins within the range of 31.2 pg/ml - 2000 pg/ml.

Sequence Length

113

Applicable Applications for S100A9 elisa kit

Sandwich ELISA (EIA)

Samples

Cell Culture Supernatants, Serum, Plasma

Assay Type

Quantitative Sandwich

Sensitivity

<10 pg/ml

Preparation and Storage

Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A9 elisa kit

Principle of the Assay: The MyBioSource' Mouse MRP14 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MRP14 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse MRP14 coated on a 96-well plate. Standards and samples are pipetted into the wells and MRP14 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse MRP14 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MRP14 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.

NCBI and Uniprot Product Information

NCBI GI #

399173

NCBI GeneID

20202

UniProt Accession #

P31725

Molecular Weight

13,049 Da

NCBI Official Full Name

Protein S100-A9

NCBI Official Synonym Full Names

S100 calcium binding protein A9 (calgranulin B)

NCBI Official Symbol

S100a9

NCBI Official Synonym Symbols

p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964

NCBI Protein Information

protein S100-A9

UniProt Protein Name

Protein S100-A9

Protein Family

Protein

UniProt Gene Name

S100a9

UniProt Synonym Gene Names

Cagb; Mrp14; MRP-14; p14

UniProt Entry Name

S10A9_MOUSE

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Cellular Component: cytoplasm; cytoskeleton; extracellular region; extracellular space; membrane; nucleus; plasma membrane

Molecular Function: antioxidant activity; arachidonic acid binding; calcium ion binding; metal ion binding; microtubule binding; RAGE receptor binding; zinc ion binding

Biological Process: actin cytoskeleton reorganization; apoptosis; astrocyte development; autophagy; caspase activation; chemotaxis; immune system process; inflammatory response; innate immune response; leukocyte chemotaxis; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of inflammatory response; regulation of integrin biosynthetic process; regulation of translation

Research Articles on S100A9

1. Alarmins S100A8/S100A9 aggravate osteophyte formation in experimental osteoarthritis and predict osteophyte progression in early human symptomatic osteoarthritis.

Similar Products

Product Notes

The Mouse S100A9 s100a9 (Catalog #AAA8244633) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244633 ELISA Kit recognizes Mouse S100A9. AAA Biotech's MRP14 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the S100A9 s100a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRP14, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Loading...

Mouse MRP14 ELISA Kit | S100A9 elisa kit