S100A9 recombinant protein

S100A9 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122663

• Gene Names: S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
• Purity: >95% as determined by SDS-PAGE
• Synonyms: S100A9; S100A9 Protein; Mouse; Recombinant (His Tag); Mouse S100A9/CAGB/p14 Protein (His Tag); 60B8Ag Protein; AW546964 Protein; BEE22 Protein; Cagb Protein; GAGB Protein; L1Ag Protein; MRP14 Protein; p14 Protein; S100 calcium binding protein A9; S100A9 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 1mM DTT, 20% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala2-Lys113

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse S100A9 (P31725)(Aal 2-Lys 113) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for S100A9 recombinant protein

Background: S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calciumbinding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Product Categories/Family for S100A9 recombinant protein

Calcium-binding Proteins and Related Molecules

References

Miyasaki KT. et al., 1993, J Dent Res. 72: 517-23. Fano G. et al., 1995, Prog Neurobiol. 46 (1): 71-82. Vogl T. et al., 2004, Blood. 104: 4260-8. Viemann D. et al., 2005, Blood. 105: 2955-62. Nakatani Y. et al., 2005, Mediators Inflamm. 2005: 280-92. Bjoerk P. et al., 2009, PLoS Biol. 7: E97-E97.

NCBI and Uniprot Product Information

• NCBI GI #: 529367291
• NCBI GeneID: 20202
• NCBI Accession #: NP_001268781.1
• NCBI GenBank Nucleotide #: NM_001281852.1
• UniProt Accession #: P31725
• Molecular Weight: 13,049 Da
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9 (calgranulin B)
• NCBI Official Symbol: S100a9
• NCBI Official Synonym Symbols: p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
• NCBI Protein Information: protein S100-A9; S100 calcium-binding protein A9; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14
• UniProt Protein Name: Protein S100-A9
• Protein Family: Protein
• UniProt Gene Name: S100a9
• UniProt Synonym Gene Names: Cagb; Mrp14; MRP-14; p14
• UniProt Entry Name: S10A9_MOUSE

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; plasma membrane; extracellular region; nucleus

Molecular Function: antioxidant activity; arachidonic acid binding; RAGE receptor binding; zinc ion binding; microtubule binding; metal ion binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; immune system process; apoptosis; leukocyte chemotaxis; positive regulation of peptide secretion; chemotaxis; leukocyte migration during inflammatory response; regulation of inflammatory response; actin cytoskeleton reorganization; innate immune response; autophagy; regulation of integrin biosynthetic process; inflammatory response; positive regulation of inflammatory response