Chicken Sp100 ELISA Kit | Sp100 elisa kit

Chicken Sp100 (Sp100 Nuclear Antigen) ELISA Kit

Cat. Num. AAA2506674

• Gene Names: SP100; lysp100b
• Reactivity: Chicken
• Synonyms: Sp100; Chicken Sp100 (Sp100 Nuclear Antigen) ELISA Kit; Sp100 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken Sp100. No significant cross-reactivity or interference between Chicken Sp100 and analogues was observed.
• Sequence Length: 10

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Sp100 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken Sp100 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Sp100. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sp100 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sp100, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sp100. You can calculate the concentration of Sp100 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1255929
• NCBI GeneID: 6672
• UniProt Accession #: P23497; O75450; Q13343; Q8TE34; Q96F70; Q96T24; Q96T95; B4DDX5; B8ZZD8; E7EUA7; E9PH61; F8WFE2
• Molecular Weight: 50,032 Da
• NCBI Official Full Name: Sp100, partial
• NCBI Official Synonym Full Names: SP100 nuclear antigen
• NCBI Official Symbol: SP100
• NCBI Official Synonym Symbols: lysp100b
• NCBI Protein Information: nuclear autoantigen Sp-100; SP100-HMG nuclear autoantigen; nuclear dot-associated Sp100 protein; speckled 100 kDa
• UniProt Protein Name: Nuclear autoantigen Sp-100
• Protein Family: Nuclear autoantigen
• UniProt Gene Name: SP100
• UniProt Entry Name: SP100_HUMAN

NCBI Description

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

Uniprot Description

SP100: May play a role in the control of gene expression. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; PML body; Mre11 complex; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein domain specific binding; protein binding; protein homodimerization activity; DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; kinase binding; transcription corepressor activity

Biological Process: retinoic acid receptor signaling pathway; negative regulation of protein export from nucleus; response to retinoic acid; transcription, DNA-dependent; viral reproduction; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; negative regulation of cell motility; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; chromatin remodeling; negative regulation of viral transcription; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; response to cytokine stimulus; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; telomere maintenance