RSPO-2 Recombinant Protein | RSPO2 recombinant protein

RSPO-2, Recombinant, Human (R-Spondin 2, Cysteine-rich and Single Thrombospondin Domain Containing Protein 2, Cristin 2) (BSA Free)

Cat. Num. AAA637830

• Gene Names: RSPO2; CRISTIN2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: RSPO-2; Recombinant; Human (R-Spondin 2; Cysteine-rich and Single Thrombospondin Domain Containing Protein 2; Cristin 2) (BSA Free); RSPO2 recombinant protein

• Reactivity: Human
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.

• Applicable Applications for RSPO2 recombinant protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA:with an apparent KD 150 nM.; Western Blot: 22.0kD migrates as an ~ 25-29kD protein in SDS-PAGE under reducing conditions.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for RSPO2 recombinant protein

Human Roof plate-specific Spondin 2 isoform 1 (R-Spondin 2, RSPO-2), also known as cysteine-rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33kD secreted protein that belongs to the R-Spondin family. The four known human R-Spondins regulate Beta-catenin signaling and overlap in expression and function (1-3). Like other R-Spondins, R-Spondin 2 contains two adjacent cysteine-rich furin-like domains (aa 90-134) followed by a thrombospondin (TSP-1) motif (aa 144-204) and a C-terminal region rich in basic residues (aa 207-243). The basic region appears to mediate cell surface retention but not to influence function (1). R-Spondin 2 contains one potential N-glycosylation site. Of the three reported splice isoforms of human R-Spondin 2, isoform 2 lacks residues 1-67 of isoform 1, while isoform 3 has a glycine substitution for residues 32-95 of isoform 1. Human R-Spondin 2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is downregulated in tumors of these tissues (1). In the embryonic mouse, R-Spondin 2 is expressed most highly in the hippocampus and in developing teeth and bones (4). Studies in Xenopus and mouse embryos indicate that R-Spondin 2 is an extracellular activator of Wnt/Beta-catenin signaling and is required for myogenesis (1). Mouse R-Spondin proteins bind both LRP-6 and Frizzled-8 but do not appear to form a ternary complex. (3) R-Spondin 2 overexpression in Xenopus also blocks signaling of TGF-Beta ligands activin, nodal and BMP-4 (1). Human R-Spondin 2 is highly conserved across species, sharing 97-98% aa identity with mouse, rat, dog, cow and opossum R-Spondin 2 and 86% aa identity with Xenopus R-Spondin 2 within aa 22-205. Mature R-Spondin 2 shares ~40% aa identity with R-Spondin 1, R-Spondin 3 and R-Spondin 4.

Product Categories/Family for RSPO2 recombinant protein

Molecular Biology; MB-Thrombospondin

NCBI and Uniprot Product Information

• NCBI GI #: 222446611
• NCBI GeneID: 340419
• NCBI Accession #: NP_848660.3
• NCBI GenBank Nucleotide #: NM_178565.4
• UniProt Accession #: Q6UXX9; Q4G0U4; Q8N6X6; B3KVP0
• Molecular Weight: 22.0kD
• NCBI Official Full Name: R-spondin-2 isoform 1
• NCBI Official Synonym Full Names: R-spondin 2
• NCBI Official Symbol: RSPO2
• NCBI Official Synonym Symbols: CRISTIN2
• NCBI Protein Information: R-spondin-2; R-spondin 2 homolog; roof plate-specific spondin-2
• UniProt Protein Name: R-spondin-2
• Protein Family: R-spondin
• UniProt Gene Name: RSPO2
• UniProt Synonym Gene Names: hRspo2
• UniProt Entry Name: RSPO2_HUMAN

NCBI Description

R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]

Uniprot Description

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding

Biological Process: embryonic forelimb morphogenesis; osteoblast differentiation; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization