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Western Blot (WB) (Western blot analysis of anti-Ihh Pab (RB10378) in HL60 cell line lysates (35ug/lane). Ihh (arrow) was detected using the purified Pab.)

Rabbit Ihh Polyclonal Antibody | anti-Q80XI9 antibody

Ihh Antibody (C-term)

Reactivity
Human, mouse (Predicted Reactivity: Chicken, Zebrafish, Xenopus)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Ihh; Polyclonal Antibody; Ihh Antibody (C-term); Indian hedgehog; anti-Q80XI9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Zebrafish, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Ihh antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-200 amino acids from the C-terminal region of human Ihh.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
169-200
Sequence Length
449
Applicable Applications for anti-Q80XI9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-Ihh Pab (RB10378) in HL60 cell line lysates (35ug/lane). Ihh (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-Ihh Pab (RB10378) in HL60 cell line lysates (35ug/lane). Ihh (arrow) was detected using the purified Pab.)
Related Product Information for anti-Q80XI9 antibody
Ihh (Indian hedgehog) is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal.
References
Kobayashi,T., Proc. Natl. Acad. Sci. U.S.A. 105 (6), 1949-1954 (2008)
Gat-Yablonski,G.,J. Pediatr. Endocrinol. Metab. 20 (11), 1215-1222 (2007)
Koyama,E., Ann. N. Y. Acad. Sci. 1116, 100-112 (2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49445
NCBI Official Full Name
indian hedgehog protein
NCBI Official Synonym Full Names
Indian hedgehog
NCBI Official Symbol
Ihh
NCBI Protein Information
indian hedgehog protein
UniProt Protein Name
Hedgehog protein
UniProt Gene Name
Ihh
UniProt Entry Name
Q80XI9_MOUSE

Uniprot Description

IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.

Cellular Component: proteinaceous extracellular matrix; membrane; plasma membrane; extracellular region

Molecular Function: peptidase activity; protein binding; hydrolase activity; metal ion binding; patched binding; calcium ion binding

Biological Process: vitelline membrane formation; proteoglycan metabolic process; multicellular organismal development; positive regulation of collagen biosynthetic process; cell maturation; cell fate specification; proteolysis; embryonic pattern specification; positive regulation of T cell differentiation in the thymus; smooth muscle development; cell-cell signaling; vasculature development; embryonic digestive tract morphogenesis; regulation of cell differentiation; regulation of growth; pancreas development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; cell communication; heart looping; cell differentiation; skeletal development; bone resorption; negative regulation of immature T cell proliferation in the thymus; smoothened signaling pathway; ossification; camera-type eye development; intein-mediated protein splicing; in utero embryonic development; multicellular organism growth; negative regulation of eye pigmentation; morphogenesis of a branching structure; negative regulation of T cell differentiation in the thymus; pattern specification process; negative regulation of signal transduction; patterning of blood vessels; osteoblast differentiation; negative regulation of cell differentiation; embryonic development; positive regulation of chondrocyte differentiation; cartilage development; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; positive regulation of alpha-beta T cell differentiation; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of alpha-beta T cell differentiation

Research Articles on Q80XI9

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Product Notes

The Q80XI9 ihh (Catalog #AAA9210445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 169-200. The Ihh Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Chicken, Zebrafish, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's Ihh can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Q80XI9 ihh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ihh, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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