RSK4 Recombinant Protein | RPS6KA6 recombinant protein

RSK4 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8120025

• Gene Names: RPS6KA6; RSK4; PP90RSK4
• Purity: >85% as determined by SDS-PAGE
• Synonyms: RSK4; RSK4 Protein; Human; Recombinant (GST Tag); Human RSK4/RPS6KA6 Protein (GST Tag); PP90RSK4 Protein; ribosomal protein S6 kinase; 90kDa; polypeptide 6; RPS6KA6 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM Reduced Glutathione, 10% gly, 0.5mM PMSF, 0.5mM EDTA, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer inf
• Sequence: Met1-Leu745

• Species: Human
• Activity: No Kinase Activity
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the full length of human RSK4 (NP_055311.1)(Met 1-Leu 745) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RPS6KA6 recombinant protein

Background: Ribosomal protein S6 kinase alpha-6, also known as Ribosomal S6 kinase 4, 9kDa ribosomal protein S6 kinase 6,RSK-4, RSK4 and RPS6KA6, is a protein that belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and S6 kinase subfamily. RPS6KA6 contains one AGC-kinase C-terminal domain and two protein kinase domains. RPS6KA6 forms a complex with either ERK1 or ERK2 in quiescent cells. RPS6KA6 shows a high level of homology to three isolated members of the human RSK family. RSK2 is involved in Coffin-Lowry syndrome and nonspecific MRX. The localization of RPS6KA6 in the interval that is commonly deleted in mentally retarded males together with the high degree of amino acid identity with RSK2 suggests that RPS6KA6 plays a role in normal neuronal development. Further mutation analyses in males with X-linked mental retardation must prove that the gene of RPS6KA6 is indeed a novel MRX gene. RPS6KA6 is a serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. RPS6KA6 is activated by multiple phosphorylations on threonine and serine residues.

Product Categories/Family for RPS6KA6 recombinant protein

EGFR Signaling Pathway

References

Yntema H.G., et al.,(1999), A novel ribosomal S6-kinase (RSK4; RPS6KA6) is commonly deleted in patients with complex X-linked mental retardation. Genomics 62:332-343.Ross M.T., et al., (2005), The DNA sequence of the human X chromosome.Nature 434:325-337.Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 7657526
• NCBI GeneID: 27330
• NCBI Accession #: NP_055311.1
• NCBI GenBank Nucleotide #: NM_014496.4
• UniProt Accession #: Q9UK32; Q6FHX2; Q8WX28; Q9H4S6; B2R854
• Molecular Weight: 83,872 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-6
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 6
• NCBI Official Symbol: RPS6KA6
• NCBI Official Synonym Symbols: RSK4; PP90RSK4
• NCBI Protein Information: ribosomal protein S6 kinase alpha-6; RSK-4; p90RSK6; p90-RSK 6; S6K-alpha 6; S6K-alpha-6; ribosomal S6 kinase 4; 90 kDa ribosomal protein S6 kinase 6
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-6
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA6
• UniProt Synonym Gene Names: RSK4; S6K-alpha-6; p90-RSK 6; p90RSK6; RSK-4
• UniProt Entry Name: KS6A6_HUMAN

NCBI Description

This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010]

Uniprot Description

RSK4: an AGC kinase of the RSK family. Phosphorylates a wide range of substrates including ribosomal protein S6. Implicated in the activation of the mitogen- activated kinase cascade. May play a role in normal neuronal development.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: Xq21

Cellular Component: nucleoplasm; mitochondrion; nucleolus; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: axon guidance; synaptic transmission; negative regulation of embryonic development; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; central nervous system development; signal transduction; protein amino acid phosphorylation