RNA (guanine-7-) Methyltransferase Recombinant Protein | RNMT recombinant protein
Recombinant Human RNA (guanine-7-) Methyltransferase
Gene Names
RNMT; MET; RG7MT1; hCMT1c
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
RNA (guanine-7-) Methyltransferase; Recombinant Human RNA (guanine-7-) Methyltransferase; RNMT Human; RNA (guanine-7-) Methyltransferase Human Recombinant; mRNA cap guanine-N7 methyltransferase; RG7MT1; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p; RNMT; KIAA0398; MET; hCMT1c; DKFZp686H1252; RNMT recombinant protein
Host
E Coli
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
RNMT solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) 2mM DTT, 20% glycerol and 100mM NaCl.
Sterile filtered colorless solution.
Sterile filtered colorless solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ
Sequence Length
476
Preparation and Storage
RNMT Human Recombinant although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.
Related Product Information for RNMT recombinant protein
Description: RNMT produced in E Coli is a single, non-glycosylated polypeptide chain containing 496 amino acids (1-476 a.a.) and having a molecular mass of 57kDa.RNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: RNMT is a widely expressed nuclear protein which is a member of the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thus producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA.
Introduction: RNMT is a widely expressed nuclear protein which is a member of the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thus producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA.
Product Categories/Family for RNMT recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,725 Da
NCBI Official Full Name
mRNA cap guanine-N7 methyltransferase
NCBI Official Synonym Full Names
RNA (guanine-7-) methyltransferase
NCBI Official Symbol
RNMT
NCBI Official Synonym Symbols
MET; RG7MT1; hCMT1c
NCBI Protein Information
mRNA cap guanine-N7 methyltransferase; hCMT1; hMet; hcm1p; mRNA (guanine-7-)methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap methyltransferase
UniProt Protein Name
mRNA cap guanine-N7 methyltransferase
UniProt Gene Name
RNMT
UniProt Synonym Gene Names
KIAA0398; hCMT1; hMet; hcm1p
UniProt Entry Name
MCES_HUMAN
Uniprot Description
RNMT: mRNA capping methyltransferase that methylates the N7 position of the added guanosine to the 5' cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT- importin alpha to bind RNA substrates.Interacts with elongating form of polymerase II and RNGTT. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase; RNA processing; EC 2.1.1.56
Chromosomal Location of Human Ortholog: 18p11.21
Cellular Component: nucleoplasm; mRNA cap complex; nucleolus; nucleus; receptor complex
Molecular Function: protein binding; RNA binding; mRNA (guanine-N7-)-methyltransferase activity
Biological Process: transcription from RNA polymerase II promoter; mRNA capping; viral reproduction; gene expression
Research Articles on RNMT
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Product Notes
The RNMT rnmt (Catalog #AAA144656) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ. It is sometimes possible for the material contained within the vial of "RNA (guanine-7-) Methyltransferase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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