Rabbit RNMT Polyclonal Antibody | anti-RNMT antibody

RNMT Polyclonal Antibody

Cat. Num. AAA3010610

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RNMT; Polyclonal Antibody; RNMT Polyclonal Antibody; cm1p; CMT1; CMT1c; hCMT1; hMet; Met; MET; RG7MT1; anti-RNMT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RNMT polyclonal antibody detects endogenous levels of RNMT protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 476

• Applicable Applications for anti-RNMT antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of mouse RNMT
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using RNMT antibody.)

Related Product Information for anti-RNMT antibody

RNMT (RNA (guanine-7-) methyltransferase), also known as MET, RG7MT1 or hCMT1c, is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. It is responsible for catalyzing the final step in the attachment of the m7GpppN cap to the 5' end of mRNA. Capping of mRNA plays an important role in mRNA processing, stability and translation and is therefore important for efficient gene expression. There are three enzymatic steps in the generation of the mRNA cap. The first two steps are catalyzed by RNGTT (RNA guanylyltransferase and 5' phosphatase), and the third step is catalyzed by RNMT. More specifically, RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 74735378
• UniProt Accession #: O43148
• Molecular Weight: 55kDa
• NCBI Official Full Name: mRNA cap guanine-N7 methyltransferase
• UniProt Protein Name: mRNA cap guanine-N7 methyltransferase
• Protein Family: RNMT-activating mini protein
• UniProt Gene Name: RNMT
• UniProt Synonym Gene Names: KIAA0398; hCMT1; hMet; hcm1p
• UniProt Entry Name: MCES_HUMAN

Uniprot Description

RNMT: mRNA capping methyltransferase that methylates the N7 position of the added guanosine to the 5' cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT- importin alpha to bind RNA substrates.Interacts with elongating form of polymerase II and RNGTT. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; RNA processing; EC 2.1.1.56

Chromosomal Location of Human Ortholog: 18p11.21

Cellular Component: nucleoplasm; mRNA cap complex; nucleolus; nucleus; receptor complex

Molecular Function: protein binding; RNA binding; mRNA (guanine-N7-)-methyltransferase activity

Biological Process: transcription from RNA polymerase II promoter; mRNA capping; viral reproduction; gene expression