Mouse anti-Human AT Rich Interactive Domain 4A Monoclonal Antibody | anti-ARID4A antibody

AT Rich Interactive Domain 4A (ARID4A, RBP1-Like)

Cat. Num. AAA600424

• Gene Names: ARID4A; RBP1; RBBP1; RBP-1; RBBP-1
• Reactivity: Human
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: AT Rich Interactive Domain 4A; Monoclonal Antibody; AT Rich Interactive Domain 4A (ARID4A; RBP1-Like); Anti -AT Rich Interactive Domain 4A (ARID4A; anti-ARID4A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C25
• Specificity: Recognizes human AT Rich Interactive Domain 4A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-ARID4A antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Synthetic peptide from the C-terminal of human ARID4A
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of immunogen using MBS600424.)

Related Product Information for anti-ARID4A antibody

AT Rich Interactive Domain 4A (ARID4A) is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity.

Product Categories/Family for anti-ARID4A antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119601138
• NCBI GeneID: 5926
• UniProt Accession #: P29374; Q15991; Q15992; Q15993; Q05CG0
• Molecular Weight: 142,752 Da
• NCBI Official Full Name: AT rich interactive domain 4A (RBP1-like), isoform CRA_c
• NCBI Official Synonym Full Names: AT rich interactive domain 4A (RBP1-like)
• NCBI Official Symbol: ARID4A
• NCBI Official Synonym Symbols: RBP1; RBBP1; RBP-1; RBBP-1
• NCBI Protein Information: AT-rich interactive domain-containing protein 4A; OTTHUMP00000179023; OTTHUMP00000179024; retinoblastoma binding protein 1; retinoblastoma-binding protein 1; ARID domain-containing protein 4A
• UniProt Protein Name: AT-rich interactive domain-containing protein 4A
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID4A
• UniProt Synonym Gene Names: RBBP1; RBP1
• UniProt Entry Name: ARI4A_HUMAN

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described. [provided by RefSeq]

Uniprot Description

RbBP1: Interacts with the viral protein-binding domain of the retinoblastoma protein. Interacts with BRMS1. Identified in mSin3A corepressor complexes together with SIN3A, SIN3B, RBBP4, RBBP7, SAP30, BRMS1, HDAC1 and HDAC2. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; erythrocyte development; establishment and/or maintenance of chromatin architecture; genetic imprinting; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; negative regulation of transcription, DNA-dependent