Ribonuclease H1 Recombinant Protein | RNASEH1 recombinant protein
Recombinant E Coli Ribonuclease H1
Gene Names
rnhA; cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Ribonuclease H1; Recombinant E Coli Ribonuclease H1; RNASEH1 E.Coli; Ribonuclease H1 E.Coli Recombinant; Ribonuclease HI; RNase HI; Ribonuclease H; RNase H; rnhA; dasF; herA; rnh; sdrA; b0214; JW0204; RNASEH1 recombinant protein
Host
E Coli
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
RNASEH1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 2mM DTT.
Sterile Filtered clear solution.
Sterile Filtered clear solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV
Sequence Length
155
Related Product Information for RNASEH1 recombinant protein
Description: RNASEH1 E Coli Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 178 amino acids (1-155 a.a.) and having a molecular mass of 20kDa.RNASEH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: RNHA is an endonuclease which specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, the RNHA protein mediates the removal of Okazaki fragment RNA primers which are present on the lagging strand during DNA replication. RNHA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is capable of binding magnesium or manganese as cofactors.
Introduction: RNHA is an endonuclease which specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, the RNHA protein mediates the removal of Okazaki fragment RNA primers which are present on the lagging strand during DNA replication. RNHA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is capable of binding magnesium or manganese as cofactors.
Product Categories/Family for RNASEH1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,597 Da
NCBI Official Full Name
ribonuclease HI, degrades RNA of DNA-RNA hybrids
NCBI Official Symbol
rnhA
NCBI Official Synonym Symbols
cer; dasF; ECK0214; herA; JW0204; rnh; sdrA; sin
NCBI Protein Information
ribonuclease HI, degrades RNA of DNA-RNA hybrids
UniProt Protein Name
Ribonuclease HI
Protein Family
UniProt Gene Name
rnhA
UniProt Synonym Gene Names
dasF; herA; rnh; sdrA; RNase HI; RNase H
UniProt Entry Name
RNH_ECOLI
NCBI Description
RNase H cleaves RNA in RNA-DNA hybrids. [More information is available at EcoCyc: EG10860].
Uniprot Description
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.
Research Articles on RNASEH1
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Product Notes
The RNASEH1 rnha (Catalog #AAA145257) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSMLK QVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV. It is sometimes possible for the material contained within the vial of "Ribonuclease H1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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