Typical Testing Data/Standard Curve (for reference only)
Human Calcium binding and coiled coil domain containing protein 2 (CALCOCO2) ELISA Kit | CALCOCO2 elisa kit
Human Calcium binding and coiled coil domain containing protein 2 (CALCOCO2) ELISA Kit
Gene Names
CALCOCO2; NDP52
Reactivity
Human
Synonyms
Calcium binding and coiled coil domain containing protein 2 (CALCOCO2); Human Calcium binding and coiled coil domain containing protein 2 (CALCOCO2) ELISA Kit; CALCOCO2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CALCOCO2. No significant cross-reactivity or interference between CALCOCO2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALCOCO2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CALCOCO2 elisa kit
Intended Uses: This CALCOCO2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCALCOCO2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CALCOCO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALCOCO2 antibody and an CALCOCO2-HRP conjugate. The assay sample and buffer are incubated together with CALCOCO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALCOCO2 concentration since CALCOCO2 from samples and CALCOCO2-HRP conjugate compete for the anti-CALCOCO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CALCOCO2 from the sample, fewer sites are left to bind CALCOCO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALCOCO2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CALCOCO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALCOCO2 antibody and an CALCOCO2-HRP conjugate. The assay sample and buffer are incubated together with CALCOCO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALCOCO2 concentration since CALCOCO2 from samples and CALCOCO2-HRP conjugate compete for the anti-CALCOCO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CALCOCO2 from the sample, fewer sites are left to bind CALCOCO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALCOCO2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CALCOCO2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,669 Da
NCBI Official Full Name
calcium-binding and coiled-coil domain-containing protein 2 isoform 3
NCBI Official Synonym Full Names
calcium binding and coiled-coil domain 2
NCBI Official Symbol
CALCOCO2
NCBI Official Synonym Symbols
NDP52
NCBI Protein Information
calcium-binding and coiled-coil domain-containing protein 2; nuclear dot protein 52; nuclear domain 10 protein 52; nuclear domain 10 protein NDP52; antigen nuclear dot 52 kDa protein
UniProt Protein Name
Calcium-binding and coiled-coil domain-containing protein 2
Protein Family
UniProt Gene Name
CALCOCO2
UniProt Synonym Gene Names
NDP52; Nuclear domain 10 protein 52
UniProt Entry Name
CACO2_HUMAN
NCBI Description
This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Uniprot Description
CALCOCO2: May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion. Part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6. Interacts with GEMIN4. Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: Golgi apparatus; cytoskeleton; intracellular membrane-bound organelle; membrane; perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: protein binding; protein homodimerization activity
Biological Process: viral reproduction
Research Articles on CALCOCO2
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Product Notes
The Human CALCOCO2 calcoco2 (Catalog #AAA7220182) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7220182 ELISA Kit recognizes Human CALCOCO2. It is sometimes possible for the material contained within the vial of "Calcium binding and coiled coil domain containing protein 2 (CALCOCO2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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