Ring Finger Protein 7 Recombinant Protein | RNF7 recombinant protein

Recombinant Human Ring Finger Protein 7

Cat. Num. AAA146475

• Gene Names: RNF7; SAG; ROC2; CKBBP1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Ring Finger Protein 7; Recombinant Human Ring Finger Protein 7; RNF7 Human; Ring Finger Protein 7 Human Recombinant; RING-box protein 2 isoform 1; Ring finger protein 7; CKBBP1; ROC2; SAG; RING-box protein 2; Rbx2; CKII beta-binding protein 1; Regulator of cullins 2; Sensitive to apoptosis gene protein; RBX2; RNF7; RNF7 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: RNF7 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK.
• Sequence Length: 97

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for RNF7 recombinant protein

Description: RNF7 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 136 amino acids (1-113 a.a) and having a molecular mass of 15.1kDa.RNF7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Ring Finger Protein 7, also known as  RNF7, is an extremely conserved ring finger protein. RNF7 is a vital subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases that are a part of the protein degradation machinery important for cell cycle progression and signal transduction. RNF7 is a substrate of casein kinase II (CSNK2A1/CKII) and also interacts with it. The phosphorylation of RNF7 by CSNK2A1 promotes the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B).

Product Categories/Family for RNF7 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Ring Finger Protein

NCBI and Uniprot Product Information

• NCBI GI #: 319004144
• NCBI GeneID: 9616
• NCBI Accession #: NP_001188299.1
• NCBI GenBank Nucleotide #: NM_001201370.1
• UniProt Accession #: Q9UBF6; Q9BXN8; Q9Y5M7; A8K1H9; A8MTB5; C9JYL3; D3DNF7; D3DNF8
• Molecular Weight: 10,876 Da
• NCBI Official Full Name: RING-box protein 2 isoform 4
• NCBI Official Synonym Full Names: ring finger protein 7
• NCBI Official Symbol: RNF7
• NCBI Official Synonym Symbols: SAG; ROC2; CKBBP1
• NCBI Protein Information: RING-box protein 2; CKII beta-binding protein 1; rbx2; regulator of cullins 2; sensitive to apoptosis gene protein; sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
• UniProt Protein Name: RING-box protein 2
• Protein Family: RING-box protein
• UniProt Gene Name: RNF7
• UniProt Synonym Gene Names: RBX2; ROC2; SAG; Rbx2; CKBBP1
• UniProt Entry Name: RBX2_HUMAN

NCBI Description

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF7: a highly conserved ring finger protein. An essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. The RING-type zinc finger recruits the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. This protein interacts with, and is a substrate of, casein kinase II (CKII). The phosphorylation of this protein by CKII has been shown to promote the degradation of IkappaB-alpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Two alternatively spliced isoforms have been reported.

Protein type: Ligase; Motility/polarity/chemotaxis; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 3q22-q24

Cellular Component: nucleoplasm; Cul5-RING ubiquitin ligase complex; cytoplasm; nucleus

Molecular Function: protein binding; copper ion binding; zinc ion binding; NEDD8 ligase activity

Biological Process: protein neddylation; protein ubiquitination; response to redox state; induction of apoptosis by oxidative stress; negative regulation of apoptosis