Rabbit anti-Human, Mouse RNF7 Polyclonal Antibody | anti-RNF7 antibody

RNF7 Antibody

Cat. Num. AAA9408070

• Gene Names: RNF7; SAG; ROC2; CKBBP1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: RNF7; Polyclonal Antibody; RNF7 Antibody; SAG; ROC2; CKBBP1; anti-RNF7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total RNF7 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 97

• Applicable Applications for anti-RNF7 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:200-1:1000; Immunohistochemistry: 1:10-1:50
• Immunogen Type: Protein
• Immunogen Description: Full length fusion protein
• Target Name: RNF7
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 12%SDS-PAGE Lysate: 40ug Mouse liver tissuePrimary antibody: 1/250 dilution Secondary antibody dilution: 1/8000Exposure time: 10 minutes)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.)

Related Product Information for anti-RNF7 antibody

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B).

Product Categories/Family for anti-RNF7 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 319004144
• NCBI GeneID: 9616
• NCBI Accession #: NP_001188299.1
• NCBI GenBank Nucleotide #: NM_001201370.1
• UniProt Accession #: Q9UBF6; Q9BXN8; Q9Y5M7; A8K1H9; A8MTB5; C9JYL3; D3DNF7; D3DNF8
• Molecular Weight: 10,876 Da
• NCBI Official Full Name: RING-box protein 2 isoform 4
• NCBI Official Synonym Full Names: ring finger protein 7
• NCBI Official Symbol: RNF7
• NCBI Official Synonym Symbols: SAG; ROC2; CKBBP1
• NCBI Protein Information: RING-box protein 2
• UniProt Protein Name: RING-box protein 2
• Protein Family: RING-box protein
• UniProt Gene Name: RNF7
• UniProt Synonym Gene Names: RBX2; ROC2; SAG; Rbx2; CKBBP1
• UniProt Entry Name: RBX2_HUMAN

NCBI Description

The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

RNF7: a highly conserved ring finger protein. An essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. The RING-type zinc finger recruits the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. This protein interacts with, and is a substrate of, casein kinase II (CKII). The phosphorylation of this protein by CKII has been shown to promote the degradation of IkappaB-alpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Two alternatively spliced isoforms have been reported.

Protein type: Motility/polarity/chemotaxis; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 3q22-q24

Cellular Component: nucleoplasm; Cul5-RING ubiquitin ligase complex; cytoplasm; nucleus

Molecular Function: protein binding; copper ion binding; zinc ion binding; NEDD8 ligase activity

Biological Process: protein neddylation; protein ubiquitination; response to redox state; induction of apoptosis by oxidative stress; negative regulation of apoptosis