Activity
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RNF34L (CARP2) Recombinant Protein | RNF34L recombinant protein
RNF34L (CARP2), Active
Applications
Western Blot
Synonyms
RNF34L (CARP2); Active; FRING; CARP2; CARP-2; RNF189; RNF34L; RIFIFYLIN; RNF34L recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant proteins stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for RNF34L recombinant protein
Ubiquitin Enzyme Assay, Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for RNF34L recombinant protein
Recombinant full-length human RNF34L was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The RNF34L gene accession number is NM_001017368.
E3 ubiquitin-protein ligase RNF34L, also named CARP2 (Caspases-8/10 Associated RING protein 2), acts as RING?domain E3 ligases, ubiquitinates apical caspases and targets them for proteasome-mediated degradation. CARP gene silencing inhibits tumor cell survival and increases cancer cell sensitivity to the death ligand or chemotherapy-induced apoptosis. CARP proteins target the tumor suppressor p53 for degradation. RNF34L also acts at the level of endocytic vesicles to limit the intensity of TNF-induced NF-kappaB activation by the regulated elimination of the adaptor protein RIP1.
E3 ubiquitin-protein ligase RNF34L, also named CARP2 (Caspases-8/10 Associated RING protein 2), acts as RING?domain E3 ligases, ubiquitinates apical caspases and targets them for proteasome-mediated degradation. CARP gene silencing inhibits tumor cell survival and increases cancer cell sensitivity to the death ligand or chemotherapy-induced apoptosis. CARP proteins target the tumor suppressor p53 for degradation. RNF34L also acts at the level of endocytic vesicles to limit the intensity of TNF-induced NF-kappaB activation by the regulated elimination of the adaptor protein RIP1.
Product Categories/Family for RNF34L recombinant protein
References
1. Liao, W. et al: CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-kappaB activation. Curr Biol. 18(9):641-649, 2008
2. McDonald, E.R. 3rd et al: Suppression of caspase-8-and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. PNAS. 20;101(16):6170-6175, 2004 br>
3. Yang, W. et al: CARPs are E3 ligases that target apical caspases and p53. Cancer Biol. Ther. 6(11):1676-1683, 2007
2. McDonald, E.R. 3rd et al: Suppression of caspase-8-and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. PNAS. 20;101(16):6170-6175, 2004 br>
3. Yang, W. et al: CARPs are E3 ligases that target apical caspases and p53. Cancer Biol. Ther. 6(11):1676-1683, 2007
NCBI and Uniprot Product Information
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Product Notes
The RNF34L (Catalog #AAA517557) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RNF34L (CARP2) can be used in a range of immunoassay formats including, but not limited to, Ubiquitin Enzyme Assay, Western Blot (WB). Researchers should empirically determine the suitability of the RNF34L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF34L (CARP2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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