SDS-Page
RNF114 recombinant protein
RNF114, 1-228aa, Human, His tag, E Coli (Denatured)
Gene Names
RNF114; ZNF313; PSORS12
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
RNF114; 1-228aa; Human; His tag; E Coli (Denatured); RING finger protein 114; PSORS12; ZNF313; RNF114 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAQQRD CGGAAQLAGP AAEADPLGRF TCPVCLEVYE KPVQVPCGHV FCSACLQECL KPKKPVCGVC RSALAPGVRA VELERQIEST ETSCHGCRKN FFLSKIRSHV ATCSKYQNYI MEGVKATIKD ASLQPRNVPN RYTFPCPYCP EKNFDQEGLV EHCKLFHSTD TKSVVCPICA SMPWGDPNYR SANFREHIQR RHRFSYDTFV DYDVDEEDMM NQVLQRSIID Q
Sequence Length
228
Applicable Applications for RNF114 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RNF114 recombinant protein
RNF114 contains 1 RING-type zinc finger. The protein is expressed in numerous tissues, including skin, CD4 lymphocytes and dendritic cells. It may play a role in spermatogenesis. Recombinant human RNF114 protein, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for RNF114 recombinant protein
References
Onoufriadis,A, et al. (2012). Invest. Dermatol. 132 (4), 1297-1299; Bijlmakers,M.J., et al. (2011) Hum. Mol. Genet. 20 (16), 3129-3137;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28.1kDa (251aa)
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF114
NCBI Official Synonym Full Names
ring finger protein 114
NCBI Official Symbol
RNF114
NCBI Official Synonym Symbols
ZNF313; PSORS12
NCBI Protein Information
E3 ubiquitin-protein ligase RNF114
UniProt Protein Name
E3 ubiquitin-protein ligase RNF114
Protein Family
UniProt Gene Name
RNF114
UniProt Synonym Gene Names
ZNF228; ZNF313
Uniprot Description
E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis.
Research Articles on RNF114
Similar Products
Product Notes
The RNF114 rnf114 (Catalog #AAA205736) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RNF114 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RNF114 rnf114 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAAQQRD CGGAAQLAGP AAEADPLGRF TCPVCLEVYE KPVQVPCGHV FCSACLQECL KPKKPVCGVC RSALAPGVRA VELERQIEST ETSCHGCRKN FFLSKIRSHV ATCSKYQNYI MEGVKATIKD ASLQPRNVPN RYTFPCPYCP EKNFDQEGLV EHCKLFHSTD TKSVVCPICA SMPWGDPNYR SANFREHIQR RHRFSYDTFV DYDVDEEDMM NQVLQRSIID Q. It is sometimes possible for the material contained within the vial of "RNF114, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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