Immunohistochemistry (IHC)
(Anti-RNF114 / ZNF313 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)
Rabbit anti-Human RNF114 / ZNF313 Polyclonal Antibody | anti-RNF114 / ZNF313 antibody
Anti-RNF114 / ZNF313 Antibody (aa1-228) IHC-plus
Gene Names
RNF114; ZNF313; PSORS12
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunoaffinity purified
Synonyms
RNF114 / ZNF313; Polyclonal Antibody; Anti-RNF114 / ZNF313 Antibody (aa1-228) IHC-plus; Rabbit Polyclonal (IgG) to Human RNF114 / ZNF313; Human RNF114 / ZNF313; PSORS12; Ring finger protein 114; Zinc finger protein 313; ZNF228; Zinc finger protein 228; anti-RNF114 / ZNF313 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human RNF114
Purity/Purification
Immunoaffinity purified
Form/Format
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Concentration
0.93 mg/ml (varies by lot)
Sequence Length
228
Applicable Applications for anti-RNF114 / ZNF313 antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC (1:100 - 1:1000)
IHC-P (7.5 ug/ml)
WB (1:500 - 1:3000)
IHC-P (7.5 ug/ml)
WB (1:500 - 1:3000)
Immunogen
RNF114 / ZNF313 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 228 of ZNF313 (SwissProt Q9Y508). Percent identity by BLAST analysis: Human (100%); Pig (96%); Bovine (95%); Mouse (93%); Rat (92%).
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 1 and 228 of ZNF313 (SwissProt Q9Y508). Percent identity by BLAST analysis: Human (100%); Pig (96%); Bovine (95%); Mouse (93%); Rat (92%).
Immunogen Type
Recombinant protein
Antigen Modification
aa1-228
Target Species
Human
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-RNF114 / ZNF313 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-RNF114 / ZNF313 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.)
Immunocytochemistry (ICC)
(Immunofluorescence of paraformaldehyde-fixed HeLa using ZNF313 antibody at 1:200 dilution.)
Immunocytochemistry (ICC)
(Immunofluorescence of paraformaldehyde-fixed HeLa using ZNF313 antibody at 1:200 dilution.)
Western Blot (WB)
(Sample (30 ug of whole cell lysate). A: Hep G2. 12 % SDS PAGE. RNF114 antibody diluted at 1:1000)
Western Blot (WB)
(Sample (30 ug of whole cell lysate). A: Hep G2. 12 % SDS PAGE. RNF114 antibody diluted at 1:1000)
Related Product Information for anti-RNF114 / ZNF313 antibody
RNF114 Antibody, PSORS12 Antibody, Ring finger protein 114 Antibody, Zinc finger protein 313 Antibody, ZNF228 Antibody, ZNF313 Antibody, Zinc finger protein 228 Antibody Description: E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,004 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase RNF114
NCBI Official Synonym Full Names
ring finger protein 114
NCBI Official Symbol
RNF114
NCBI Official Synonym Symbols
ZNF313; PSORS12
NCBI Protein Information
E3 ubiquitin-protein ligase RNF114
UniProt Protein Name
E3 ubiquitin-protein ligase RNF114
UniProt Gene Name
RNF114
UniProt Synonym Gene Names
ZNF228; ZNF313
UniProt Entry Name
RN114_HUMAN
Uniprot Description
ZNF313: May play a role in spermatogenesis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Transcription factor
Chromosomal Location of Human Ortholog: 20q13.13
Cellular Component: cytoplasm; intracellular; nucleus
Molecular Function: ligase activity; ubiquitin conjugating enzyme binding; zinc ion binding
Biological Process: cell differentiation; multicellular organismal development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; spermatogenesis
Research Articles on RNF114 / ZNF313
Similar Products
Product Notes
The RNF114 / ZNF313 rnf114 (Catalog #AAA249973) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RNF114 / ZNF313 Antibody (aa1-228) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF114 / ZNF313 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB). ICC (1:100 - 1:1000) IHC-P (7.5 ug/ml) WB (1:500 - 1:3000). Researchers should empirically determine the suitability of the RNF114 / ZNF313 rnf114 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF114 / ZNF313, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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