Testing Data
(Peptide Competition: HeLa cell lysate left untreated (5) or phosphorylated in vitro by PAK (1-4, 6) were incubated with 0.50ug/ml MBS631030 following prior incubation with: no peptide (1, 5, 6), the non-phosphopeptide corresponding to the immu)
Rabbit anti-Chicken Myosin Light Chain Polyclonal Antibody | anti-MYL1 antibody
Myosin Light Chain, Phosphorylated (Ser19)
Gene Names
MYL1; MLC
Reactivity
Chicken
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Myosin Light Chain; Polyclonal Antibody; Phosphorylated (Ser19); Anti -Myosin Light Chain; anti-MYL1 antibody
Host
Rabbit
Reactivity
Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes chicken MLC when phosphorylated at Ser19. Species Sequence Homology: human, 100%
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3 1mg/ml BSA (IgG and protease free), 0.05% sodium azide.
Applicable Applications for anti-MYL1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Prior Immunoprecipitation of MLC may be necessary.
Dilution: Western Blot: 1:1000. Prior Immunoprecipitation of MLC may be necessary.
Immunogen
Synthetic phosphopeptide corresponding to the region of MLC that contains serine 19 (serine 20 including the initiating methionine).
Positive Control
Chicken gizzard myosin II phosphorylated in vitro by PAK.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Peptide Competition: HeLa cell lysate left untreated (5) or phosphorylated in vitro by PAK (1-4, 6) were incubated with 0.50ug/ml MBS631030 following prior incubation with: no peptide (1, 5, 6), the non-phosphopeptide corresponding to the immu)
Testing Data
(Peptide Competition: HeLa cell lysate left untreated (5) or phosphorylated in vitro by PAK (1-4, 6) were incubated with 0.50ug/ml MBS631030 following prior incubation with: no peptide (1, 5, 6), the non-phosphopeptide corresponding to the immu)
Related Product Information for anti-MYL1 antibody
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g., myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.
Product Categories/Family for anti-MYL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
myosin light chain 1/3, skeletal muscle isoform
NCBI Official Symbol
MYL1
NCBI Official Synonym Symbols
MLC
NCBI Protein Information
myosin light chain 1/3, skeletal muscle isoform; fast myosin light chain 1; Ca2+-binding protein-like protein; alkali myosin light chain 3 A2 catalytic
Protein Family
Research Articles on MYL1
Similar Products
Product Notes
The MYL1 (Catalog #AAA631030) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Myosin Light Chain, Phosphorylated (Ser19) reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin Light Chain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Prior Immunoprecipitation of MLC may be necessary. Researchers should empirically determine the suitability of the MYL1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin Light Chain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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